Maria Laura Coluccio, Gerardo Perozziello, Natalia Malara, Elvira Parrotta, Peng Zhang, Francesco Gentile, Tania Limongi, Pushparani Michael Raj, Gianni Cuda,Patrizio Candeloro, Enzo Di Fabrizio
This review covers several aspects of microfluidic devices used for culturing and monitoring of both adherent and non-adherent cells, including a multitude of applications. A comparison of available platforms with high throughput analysis, automation capability, interface to sensors and integration, is reported. Aspects, such as operational versatility of the devices, are scrutinized in terms of their analytical efficacy. It is found that due to multi-functionality capability of modern microfluidics, there is big amount of experimental data obtainable from a single device, allowing complex experimental control and efficient data correlation, particularly important when biomedical studies are considered. Hence several examples on cell culture and monitoring are given in this review, including details on design of microfluidic devices with their distinctive technological peculiarities.
DOI
Showing posts with label Microelectronic Engineering. Show all posts
Showing posts with label Microelectronic Engineering. Show all posts
Tuesday, February 19, 2019
Saturday, March 15, 2014
Monolithic integration of DUV-induced waveguides into plastic microfluidic chip for optical manipulation
M. Khoury, C. Vannahme, K.T. Sørensen, A. Kristensen, K. Berg-Sørensen
A monolithic polymer optofluidic chip for manipulation of microbeads in flow is demonstrated. On this chip, polymer waveguides induced by Deep UV lithography are integrated with microfluidic channels. The optical propagation losses of the waveguides are measured to be 0.66±0.130.66±0.13 dB/mm at a wavelength of λλ = 808 nm. An optimized bead tracking algorithm is implemented, allowing for determination of the optical forces acting on the particles. The algorithm features a spatio-temporal mapping of coordinates for uniting partial trajectories, without increased processing time. With an external laser power of 250 mW, a maximum scattering force of 0.84 pN is achieved for 5 μm diameter polystyrene beads in water.
DOI
A monolithic polymer optofluidic chip for manipulation of microbeads in flow is demonstrated. On this chip, polymer waveguides induced by Deep UV lithography are integrated with microfluidic channels. The optical propagation losses of the waveguides are measured to be 0.66±0.130.66±0.13 dB/mm at a wavelength of λλ = 808 nm. An optimized bead tracking algorithm is implemented, allowing for determination of the optical forces acting on the particles. The algorithm features a spatio-temporal mapping of coordinates for uniting partial trajectories, without increased processing time. With an external laser power of 250 mW, a maximum scattering force of 0.84 pN is achieved for 5 μm diameter polystyrene beads in water.
DOI
Subscribe to:
Comments (Atom)