We propose to reduce the photodamage in optical trapping of living cells by using azimuthally polarized beams (APBs). APBs achieve higher axial trapping efficiency and lower photodamage than linearly polarized Gaussian beam when used for optical trapping of individual red blood cells (RBCs). In particular, we further achieve reducing photodamage in optical trapping of individual RBCs in living Zebrafishes. Our work is expected to benefit in-vivo optical trapping and the study of living cells.
Showing posts with label Applied Physics Express. Show all posts
Showing posts with label Applied Physics Express. Show all posts
Tuesday, February 18, 2020
Reducing photodamage in optical trapping of individual cells in living zebrafish
Panpan Yu, Yifan Liu, Qian Zhao, Ziqiang Wang, Yinmei Li and Lei Gong
We propose to reduce the photodamage in optical trapping of living cells by using azimuthally polarized beams (APBs). APBs achieve higher axial trapping efficiency and lower photodamage than linearly polarized Gaussian beam when used for optical trapping of individual red blood cells (RBCs). In particular, we further achieve reducing photodamage in optical trapping of individual RBCs in living Zebrafishes. Our work is expected to benefit in-vivo optical trapping and the study of living cells.
We propose to reduce the photodamage in optical trapping of living cells by using azimuthally polarized beams (APBs). APBs achieve higher axial trapping efficiency and lower photodamage than linearly polarized Gaussian beam when used for optical trapping of individual red blood cells (RBCs). In particular, we further achieve reducing photodamage in optical trapping of individual RBCs in living Zebrafishes. Our work is expected to benefit in-vivo optical trapping and the study of living cells.
Tuesday, November 19, 2019
Optical fiber-based manipulation of microparticles in microfluidic channel through thermal convection
Wei Zhan, Mingjun Yang and Wuzhou Song
We demonstrate a simple optical fiber microfluidic control device based on photothermal effect induced convection, which can realize flexible manipulation and particle sorting over a range of hundreds of microns. Three single-mode optical fibers in a regular arrangement were utilized for the realization of the particle capture function, which simplifies the complex equipment and reduces the cost in traditional optical tweezers. We also display a unique control mode: the horizontal manipulation of particles by moving the fiber vertically. It will hopefully be applied in biomedical, chemical analysis, material testing and other fields.
DOI
We demonstrate a simple optical fiber microfluidic control device based on photothermal effect induced convection, which can realize flexible manipulation and particle sorting over a range of hundreds of microns. Three single-mode optical fibers in a regular arrangement were utilized for the realization of the particle capture function, which simplifies the complex equipment and reduces the cost in traditional optical tweezers. We also display a unique control mode: the horizontal manipulation of particles by moving the fiber vertically. It will hopefully be applied in biomedical, chemical analysis, material testing and other fields.
DOI
Wednesday, October 16, 2019
In-situ reflection imaging and microspectroscopic study on three-dimensional crystal growth of L-phenylalanine under laser trapping
Jim Jui-Kai Chen, Ken-ichi Yuyama, Teruki Sugiyama and Hiroshi Masuhara
We investigated growth behavior of an L-phenylalanine crystal formed by laser trapping with the use of reflection imaging and microspectroscopy. Optical reflection micrographs show colored images of the crystal due to constructive interference of incident white light. The color distribution on the crystal is dynamically changed under laser trapping, which is in addition to enlargement of the crystal plane area. The temporal change in the crystal thickness is examined by measuring reflection spectra of the crystal. We discuss the three-dimensional crystal growth under laser trapping condition by comprehensively considering the changes in the crystal thickness and the crystal plane area.
DOI
We investigated growth behavior of an L-phenylalanine crystal formed by laser trapping with the use of reflection imaging and microspectroscopy. Optical reflection micrographs show colored images of the crystal due to constructive interference of incident white light. The color distribution on the crystal is dynamically changed under laser trapping, which is in addition to enlargement of the crystal plane area. The temporal change in the crystal thickness is examined by measuring reflection spectra of the crystal. We discuss the three-dimensional crystal growth under laser trapping condition by comprehensively considering the changes in the crystal thickness and the crystal plane area.
DOI
Thursday, October 10, 2019
Optical tweezers assisted controllable formation and precise manipulation of microdroplet
Shuai Li, Chunguang Hu, Xiaoqing Gao, Guoteng Ma, Hongbin Li, Xiaodong Hu and Xiaotang Hu
We demonstrate an optical method to realize controllable formation and precise manipulation of microdroplet using optical tweezers (OT). With the irradiation of a highly focused laser into the mixture of inorganic phosphate buffered saline (PBS) and organic solvent isopropanol, a microdroplet was gradually formed at the center of the trap. The size and the growth rate of the microdroplet could be precisely controlled by regulating the laser power and the proportion of two solvents in the mixture. Furthermore, the microdroplet could be manipulated by OT to build microstructures on the slide. We also discuss the possible mechanism behind our observations and the potential usage of such discoveries.
DOI
We demonstrate an optical method to realize controllable formation and precise manipulation of microdroplet using optical tweezers (OT). With the irradiation of a highly focused laser into the mixture of inorganic phosphate buffered saline (PBS) and organic solvent isopropanol, a microdroplet was gradually formed at the center of the trap. The size and the growth rate of the microdroplet could be precisely controlled by regulating the laser power and the proportion of two solvents in the mixture. Furthermore, the microdroplet could be manipulated by OT to build microstructures on the slide. We also discuss the possible mechanism behind our observations and the potential usage of such discoveries.
DOI
Tuesday, July 23, 2019
Strong light confinement and gradient force in parallel infinite-width monolayer graphene pairs
Chunyu Lu, Zheng-Da Hu, Jin Cui, Jicheng Wang and Liang Pan
We study high light confinement, field enhancement, and strong gradient force between graphene nanoribbons. Influence of the wavelength of incident light on the gradient force, which is rare in previous studies, is presented. Results of light field confinement are achieved, which are much better than those in the slot waveguides based on artificial hyperbolic metamaterials. The propagation length and enhancement ratio can be up to 79.5 μm and 16, respectively. It is worth mentioning that the light confinement ratio can reach an astonishing 97%. Finally, we analyze the gradient force of different intensity in terms of the electric field.
DOI
We study high light confinement, field enhancement, and strong gradient force between graphene nanoribbons. Influence of the wavelength of incident light on the gradient force, which is rare in previous studies, is presented. Results of light field confinement are achieved, which are much better than those in the slot waveguides based on artificial hyperbolic metamaterials. The propagation length and enhancement ratio can be up to 79.5 μm and 16, respectively. It is worth mentioning that the light confinement ratio can reach an astonishing 97%. Finally, we analyze the gradient force of different intensity in terms of the electric field.
DOI
Monday, June 17, 2019
Position detection using differential signals of the coupling light in a tapered-lensed dual-beam optical fiber trap
Wei Xiong, Guangzong Xiao, Ying Zhang, Xiang Han, Xinlin Chen and Hui Luo
A technique for position detection in a tapered-lensed dual-beam optical fiber trap based on differential measurements is proposed. The difference in the coupling power of two tapered fibers is used to sense the position of the trapped particle. The detecting resolution can reach 45 nm. The fiber coupling theory is used to analyze the coupling efficiency of the fibers. Results show that tapered fibers are more beneficial than cleaved fibers in the differential measurements system. The influences of the radius of the taper end and the fiber separations are discussed in detail.
DOI
A technique for position detection in a tapered-lensed dual-beam optical fiber trap based on differential measurements is proposed. The difference in the coupling power of two tapered fibers is used to sense the position of the trapped particle. The detecting resolution can reach 45 nm. The fiber coupling theory is used to analyze the coupling efficiency of the fibers. Results show that tapered fibers are more beneficial than cleaved fibers in the differential measurements system. The influences of the radius of the taper end and the fiber separations are discussed in detail.
DOI
Wednesday, August 1, 2018
Bubble generation and molecular crystallization at solution surface by intense continuous-wave laser irradiation
Jim Jui-Kai Chen, Ken-ichi Yuyama, Teruki Sugiyama and Hiroshi Masuhara
We demonstrate bubble generation outside the focus induced by irradiating a focused 1064 nm continuous-wave laser beam into the surface of water and l-phenylalanine H2O solutions. In the former case of water, bubbles stay at positions distant from the focus during the irradiation, and their size and location are controllable by the laser power. In the latter solution, bubbles move outward toward the surrounding area, and subsequently crystallization takes place at the focus. We discuss these behaviors from the viewpoints of the temperature elevation accompanying the decrease in air solubility as well as the optical trapping of l-phenylalanine clusters giving a single crystal.
DOI
We demonstrate bubble generation outside the focus induced by irradiating a focused 1064 nm continuous-wave laser beam into the surface of water and l-phenylalanine H2O solutions. In the former case of water, bubbles stay at positions distant from the focus during the irradiation, and their size and location are controllable by the laser power. In the latter solution, bubbles move outward toward the surrounding area, and subsequently crystallization takes place at the focus. We discuss these behaviors from the viewpoints of the temperature elevation accompanying the decrease in air solubility as well as the optical trapping of l-phenylalanine clusters giving a single crystal.
DOI
Tuesday, May 15, 2018
Particle trapping and manipulation using hollow beam with tunable size generated by thermal nonlinear optical effect
Bo He, Xuemei Cheng, Hui Zhang, Haowei Chen, Qian Zhang, Zhaoyu Ren, Shan Ding and Jintao Ba
We report micron-sized particle trapping and manipulation using a hollow beam of tunable size, which was generated by cross-phase modulation via the thermal nonlinear optical effect in an ethanol medium. The results demonstrated that the particle can be trapped stably in air for hours and manipulated in millimeter range with micrometer-level accuracy by modulating the size of the hollow beam. The merits of flexibility in tuning the beam size and simplicity in operation give this method great potential for the in situ study of individual particles in air.
DOI
We report micron-sized particle trapping and manipulation using a hollow beam of tunable size, which was generated by cross-phase modulation via the thermal nonlinear optical effect in an ethanol medium. The results demonstrated that the particle can be trapped stably in air for hours and manipulated in millimeter range with micrometer-level accuracy by modulating the size of the hollow beam. The merits of flexibility in tuning the beam size and simplicity in operation give this method great potential for the in situ study of individual particles in air.
DOI
Friday, October 12, 2012
Optical Trapping in Air of an Individual Nanotube-Sphere Device
Gurpreet Singh, Andrew Slifka, Paul Rice, Damian Lauria, and Roop L. Mahajan
We demonstrated the optical manipulation of a polystyrene bead supported in air by an individual carbon nanotube. We have also utilized this technique to demonstrate the calibration of a nanotube-sphere force sensor in the ≈10-10 N range. A focused IR laser (at 1.064 µm, 100 mW power) was used to trap the bead. This simple device consisted of a tungsten probe with a long nanotube (length, ≥15 µm) attached to its tip, while the other end of the nanotube supported a polystyrene microsphere. Decreasing the nanotube length to 8 µm did not show any sphere motion in the trap.
DOI
We demonstrated the optical manipulation of a polystyrene bead supported in air by an individual carbon nanotube. We have also utilized this technique to demonstrate the calibration of a nanotube-sphere force sensor in the ≈10-10 N range. A focused IR laser (at 1.064 µm, 100 mW power) was used to trap the bead. This simple device consisted of a tungsten probe with a long nanotube (length, ≥15 µm) attached to its tip, while the other end of the nanotube supported a polystyrene microsphere. Decreasing the nanotube length to 8 µm did not show any sphere motion in the trap.
DOI
Thursday, May 3, 2012
Optical Tweezers with Assistance of Sub-Microsecond-Duration Pulse Laser Beam
Saki Maeda, Tadao Sugiura, and Kotaro Minato
We report optical tweezers with the assistance of a strong instantaneous force generated by a focused pulse laser beam of sub-microsecond duration. A strong instantaneous force is required in biological applications. A suitable pulse duration for pulse assistance in water is derived on the order of 100 to 1000 ns from motion analysis of a micrometer-sized particle. We performed optical tweezers experiments with a focused pulse beam of 160 ns duration coaxially incident with a CW laser beam. From the experiments on particle extraction from a glass surface, the required energy for extraction is smaller than the case of 150 µs duration by a factor of 60.
DOI
We report optical tweezers with the assistance of a strong instantaneous force generated by a focused pulse laser beam of sub-microsecond duration. A strong instantaneous force is required in biological applications. A suitable pulse duration for pulse assistance in water is derived on the order of 100 to 1000 ns from motion analysis of a micrometer-sized particle. We performed optical tweezers experiments with a focused pulse beam of 160 ns duration coaxially incident with a CW laser beam. From the experiments on particle extraction from a glass surface, the required energy for extraction is smaller than the case of 150 µs duration by a factor of 60.
DOI
Subscribe to:
Comments (Atom)