Dekai Zhou, Yuan Gao, Junjie Yang, Yuguang C. Li, Guangbin Shao, Guangyu Zhang, Tianlong Li, Longqiu Li
It is of great interest and big challenge to control the collective behaviors of nanomotors to mimic the aggregation/separation behavior of biological systems. Here, a light‐acoustic combined method is proposed to control the aggregation/separation of artificial nanomotors. It is shown that nanomotors aggregate at the pressure node in acoustic field and afterward present a collective “firework” separation behavior induced by light irradiation. The collective behavior is found to be applicable for metallic materials and polymers even different light wavelengths are used. Physical insights on the collective firework behavior resulting from the change of acoustic streaming caused by optical force are provided. It is found that diffusion velocity and diffusion region of cluster can be controlled by adjusting light intensity and acoustic excitation voltage, and irradiation direction, respectively. This harmless, controllable, and widely applicable method provides new possibilities for groups of nanomachines, drug release, and cargo transport in nanomedicine and nanosensors.
DOI
Showing posts with label Advanced Science. Show all posts
Showing posts with label Advanced Science. Show all posts
Tuesday, June 5, 2018
Friday, October 21, 2016
Detecting Swelling States of Red Blood Cells by “Cell–Fluid Coupling Spectroscopy”
Carla Zensen, Isis E. Fernandez, Oliver Eickelberg, Jochen Feldmann, Theobald Lohmüller
Red blood cells are “shaken” with a holographic optical tweezer array. The flow generated around cells due to the periodic optical forcing is measured with an optically trapped “detector” particle located in the cell vicinity. A signal-processing model that describes the cell's physical properties as an analog filter illustrates how cells can be distinguished from each other.
DOI
Red blood cells are “shaken” with a holographic optical tweezer array. The flow generated around cells due to the periodic optical forcing is measured with an optically trapped “detector” particle located in the cell vicinity. A signal-processing model that describes the cell's physical properties as an analog filter illustrates how cells can be distinguished from each other.
DOI
Thursday, September 22, 2016
Guidable Thermophoretic Janus Micromotors Containing Gold Nanocolorifiers for Infrared Laser Assisted Tissue Welding
Wenping He, Johannes Frueh, Narisu Hu, Liping Liu, Meiyu Gai, Qiang He
Current wound sealing systems such as nanoparticle-based gluing of tissues allow almost immediate wound sealing. The assistance of a laser beam allows the wound sealing with higher controllability due to the collagen fiber melting which is defined by loss of tertiary protein structure and restoration upon cooling. Usually one employs dyes to paint onto the wound, if water absorption bands are absent. In case of strong bleeding or internal wounds such applications are not feasible due to low welding depth in case of water absorption bands, dyes washing off, or the dyes becoming diluted within the wound. One possible solution of these drawbacks is to use autonomously movable particles composing of biocompatible gold and magnetite nanoparticles and biocompatible polyelectrolyte complexes. In this paper a proof of principle study is presented on the utilization of thermophoretic Janus particles and capsules employed as dyes for infrared laser-assisted tissue welding. This approach proves to be efficient in sealing the wound on the mouse in vivo. The temperature measurement of single particle level proves successful photothermal heating, while the mechanical characterizations of welded liver, skin, and meat confirm mechanical restoration of the welded biological samples.
DOI
Current wound sealing systems such as nanoparticle-based gluing of tissues allow almost immediate wound sealing. The assistance of a laser beam allows the wound sealing with higher controllability due to the collagen fiber melting which is defined by loss of tertiary protein structure and restoration upon cooling. Usually one employs dyes to paint onto the wound, if water absorption bands are absent. In case of strong bleeding or internal wounds such applications are not feasible due to low welding depth in case of water absorption bands, dyes washing off, or the dyes becoming diluted within the wound. One possible solution of these drawbacks is to use autonomously movable particles composing of biocompatible gold and magnetite nanoparticles and biocompatible polyelectrolyte complexes. In this paper a proof of principle study is presented on the utilization of thermophoretic Janus particles and capsules employed as dyes for infrared laser-assisted tissue welding. This approach proves to be efficient in sealing the wound on the mouse in vivo. The temperature measurement of single particle level proves successful photothermal heating, while the mechanical characterizations of welded liver, skin, and meat confirm mechanical restoration of the welded biological samples.
DOI
Wednesday, July 22, 2015
Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
Xi Zhao, Xiaomeng Huang, Xinmei Wang, Yun Wu, Ann-Kathrin Eisfeld, Sebastian Schwind, Daniel Gallego-Perez, Pouyan E. Boukany, Guido I. Marcucci and Ly James Lee
A comprehensive micro/nanofluidics platform for single-cell analysis based on nanochannel electroporation (NEP) and molecular beacon (MB) is presented in this study. The platform can quantitatively analyze multiple RNA species in individual cells with minimal cell damage. Furthermore, it is capable of delivering nucleic acids into target cells and subsequently detecting their responses at RNA level, e.g., microRNA (miRNA). It is known that as the downstream targets of miR-29b, DNMT3A/B can be downregulated by miR-29b overexpression. To demonstrate the activity of delivered miR-29b by NEP and the analytical function of the platform, the decreased expression of DNMT3A/B in acute myeloid leukemia (AML) cells was verified at single-cell level by simultaneous detection of multiple genes in the same cell. The potential of such platform on intracellular pathway studies has also been explored by investigating the upregulation efficiencies of miR-181a through different pathways in AML cells. The results showed that an indirect approach by C/EBPα-p30 peptide expression would have a stronger effect than direct transfection of the miR-181a gene. The platform has also shown its advantages over established technologies in the analysis of cells that are hard to transfect.
DOI
A comprehensive micro/nanofluidics platform for single-cell analysis based on nanochannel electroporation (NEP) and molecular beacon (MB) is presented in this study. The platform can quantitatively analyze multiple RNA species in individual cells with minimal cell damage. Furthermore, it is capable of delivering nucleic acids into target cells and subsequently detecting their responses at RNA level, e.g., microRNA (miRNA). It is known that as the downstream targets of miR-29b, DNMT3A/B can be downregulated by miR-29b overexpression. To demonstrate the activity of delivered miR-29b by NEP and the analytical function of the platform, the decreased expression of DNMT3A/B in acute myeloid leukemia (AML) cells was verified at single-cell level by simultaneous detection of multiple genes in the same cell. The potential of such platform on intracellular pathway studies has also been explored by investigating the upregulation efficiencies of miR-181a through different pathways in AML cells. The results showed that an indirect approach by C/EBPα-p30 peptide expression would have a stronger effect than direct transfection of the miR-181a gene. The platform has also shown its advantages over established technologies in the analysis of cells that are hard to transfect.
DOI
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