From Far-Field to Near-Field Micro- and Nanoparticle Optical Trapping

Theodoros D. Bouloumis and Síle Nic Chormaic

Optical tweezers are a very well-established technique that have developed into a standard tool for trapping and manipulating micron and submicron particles with great success in the last decades. Although the nature of light enforces restrictions on the minimum particle size that can be efficiently trapped due to Abbe’s diffraction limit, scientists have managed to overcome this problem by engineering new devices that exploit near-field effects. Nowadays, metallic nanostructures can be fabricated which, under laser illumination, produce a secondary plasmonic field that does not suffer from the diffraction limit. This advance offers a great improvement in nanoparticle trapping, as it relaxes the trapping requirements compared to conventional optical tweezers although problems may arise due to thermal heating of the metallic nanostructures. This could hinder efficient trapping and damage the trapped object. In this work, we review the fundamentals of conventional optical tweezers, the so-called plasmonic tweezers, and related phenomena. Starting from the conception of the idea by Arthur Ashkin until recent improvements and applications, we present the principles of these techniques along with their limitations. Emphasis in this review is on the successive improvements of the techniques and the innovative aspects that have been devised to overcome some of the main challenges.

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Influence of Laser Intensity Fluctuation on Single-Cesium Atom Trapping Lifetime in a 1064-nm Microscopic Optical Tweezer

Rui Sun, Xin Wang, Kong Zhang, Jun He and Junmin Wang

An optical tweezer composed of a strongly focused single-spatial-mode Gaussian beam of a red-detuned 1064-nm laser can confine a single-cesium (Cs) atom at the strongest point of the light intensity. We can use this for coherent manipulation of single-quantum bits and single-photon sources. The trapping lifetime of the atoms in the optical tweezers is very short due to the impact of the background atoms, the parametric heating of the optical tweezer and the residual thermal motion of the atoms. In this paper, we analyzed the influence of the background pressure, the trap frequency of optical tweezers and the laser intensity fluctuation of optical tweezers on the atomic trapping lifetime. Combined with the external feedback loop based on an acousto-optical modulator (AOM), the intensity fluctuation of the 1064-nm laser in the time domain was suppressed from ±3.360% to ±0.064%, and the suppression bandwidth in the frequency domain reached approximately 33 kHz. The trapping lifetime of a single-Cs atom in the microscopic optical tweezers was extended from 4.04 s to 6.34 s.

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Optical Trapping, Sizing, and Probing Acoustic Modes of a Small Virus

Jeffrey Burkhartsmeyer, Yanhong Wang, Kam Sing Wong and Reuven Gordon
Prior opto-mechanical techniques to measure vibrational frequencies of viruses work on large ensembles of particles, whereas, in this work, individually trapped viral particles were studied. Double nanohole (DNH) apertures in a gold film were used to achieve optical trapping of one of the smallest virus particles yet reported, PhiX174, which has a diameter of 25 nm. When a laser was focused onto these DNH apertures, it created high local fields due to plasmonic enhancement, which allowed stable trapping of small particles for prolonged periods at low powers. Two techniques were performed to characterize the virus particles. The particles were sized via an established autocorrelation analysis technique, and the acoustic modes were probed using the extraordinary acoustic Raman (EAR) method. The size of the trapped particle was determined to be 25 ± 3.8 nm, which is in good agreement with the established diameter of PhiX174. A peak in the EAR signal was observed at 32 GHz, which fits well with the predicted value from elastic theory.

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Plasmonic Tweezers towards Biomolecular and Biomedical Applications

Xue Han and Changsen Sun

With the capability of confining light into subwavelength scale, plasmonic tweezers have been used to trap and manipulate nanoscale particles. It has huge potential to be utilized in biomolecular research and practical biomedical applications. In this short review, plasmonic tweezers based on nano-aperture designs are discussed. A few challenges should be overcome for these plasmonic tweezers to reach a similar level of significance as the conventional optical tweezers.

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Automated Indirect Transportation of Biological Cells with Optical Tweezers and a 3D Printed Microtool

Songyu Hu, Heng Xie, Tanyong Wei, Shuxun Chen and Dong Sun

Optical tweezers are widely used for noninvasive and precise micromanipulation of living cells to understand biological processes. By focusing laser beams on cells, direct cell manipulation with optical tweezers can achieve high precision and flexibility. However, direct exposure to the laser beam can lead to negative effects on the cells. These phenomena are also known as photobleaching and photodamage. In this study, we proposed a new indirect cell micromanipulation approach combined with a robot-aided holographic optical tweezer system and 3D nano-printed microtool. The microtool was designed with a V-shaped head and an optical handle part. The V-shaped head can push and trap different sizes of cells as the microtool moves forward by optical trapping of the handle part. In this way, cell exposure to the laser beam can be effectively reduced. The microtool was fabricated with a laser direct writing system by two-photon photopolymerization. A control strategy combined with an imaging processing algorithm was introduced for automated manipulation of the microtool and cells. Experiments were performed to verify the effectiveness of our approach. First, automated microtool transportation and rotation were demonstrated with high precision. Second, indirect optical transportations of cells, with and without an obstacle, were performed to demonstrate the effectiveness of the proposed approach. Third, experiments of fluorescent cell manipulation were performed to confirm that, indicated by the photobleaching effect, indirect manipulation with the microtool could induce less laser exposure compared with direct optical manipulation. The proposed method could be useful in complex biomedical applications where precise cell manipulation and less laser exposure are required.

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Optical Conveyor Belts for Chiral Discrimination: Influence of De-Phasing Parameter

Luis Carretero, Pablo Acebal and Salvador Blaya

A numerical analysis is carried out of the influence of the de-phasing parameter of an optical conveyor belt in the enantiomeric separation. The optical conveyor belt is obtained by the interference of a Laguerre Gaussian and a Gaussian beam with different beam waists, which are temporally de-phased. In order to obtain the maximum separation distance between enantiomers, we calculate the optimum range of values of the de-phasing parameter.

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Optical Assembling of Micro-Particles at a Glass–Water Interface with Diffraction Patterns Caused by the Limited Aperture of Objective

Min-Cheng Zhong, Ai-Yin Liu and Rong Zhu

Optical tweezers can manipulate micro-particles, which have been widely used in various applications. Here, we experimentally demonstrate that optical tweezers can assemble the micro-particles to form stable structures at the glass–solution interface in this paper. Firstly, the particles are driven by the optical forces originated from the diffraction fringes, which of the trapping beam passing through an objective with limited aperture. The particles form stable ring structures when the trapping beam is a linearly polarized beam. The particle distributions in the transverse plane are affected by the particle size and concentration. Secondly, the particles form an incompact structure as two fan-shaped after the azimuthally polarized beam passing through a linear polarizer. Furthermore, the particles form a compact structure when a radially polarized beam is used for trapping. Thirdly, the particle patterns can be printed steady at the glass surface in the salt solution. At last, the disadvantage of diffraction traps is discussed in application of optical tweezers. The aggregation of particles at the interfaces seriously affects the flowing of particles in microfluidic channels, and a total reflector as the bottom surface of sample cell can avoid the optical tweezers induced particle patterns at the interface. The optical trapping study utilizing the diffraction gives an interesting method for binding and assembling microparticles, which is helpful to understand the principle of optical tweezers.

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Microfluidic Technology for Cell Manipulation

Jae-Sung Kwon and Je Hoon Oh

Microfluidic techniques for cell manipulation have been constantly developed and integrated into small chips for high-performance bioassays. However, the drawbacks of each of the techniques often hindered their further advancement and their wide use in biotechnology. To overcome this difficulty, an examination and understanding of various aspects of the developed manipulation techniques are required. In this review, we provide the details of primary microfluidic techniques that have received much attention for bioassays. First, we introduce the manipulation techniques using a sole driving source, i.e., dielectrophoresis, electrophoresis, optical tweezers, magnetophoresis, and acoustophoresis. Next, we present rapid electrokinetic patterning, a hybrid opto-electric manipulation technique developed recently. It is introduced in detail along with the underlying physical principle, operating environment, and current challenges. This paper will offer readers the opportunity to improve existing manipulation techniques, suggest new manipulation techniques, and find new applications in biotechnology. View Full-Text

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An Accurate Perception Method for Low Contrast Bright Field Microscopy in Heterogeneous Microenvironments

Keshav Rajasekaran, Ekta Samani, Manasa Bollavaram, John Stewart and Ashis G. Banerjee

Automated optical tweezers-based robotic manipulation of microscale objects requires real-time visual perception for estimating the states, i.e., positions and orientations, of the objects. Such visual perception is particularly challenging in heterogeneous environments comprising mixtures of biological and colloidal objects, such as cells and microspheres, when the popular imaging modality of low contrast bright field microscopy is used. In this paper, we present an accurate method to address this challenge. Our method combines many well-established image processing techniques such as blob detection, histogram equalization, erosion, and dilation with a convolutional neural network in a novel manner. We demonstrate the effectiveness of our processing pipeline in perceiving objects of both regular and irregular shapes in heterogeneous microenvironments of varying compositions. The neural network, in particular, helps in distinguishing the individual microspheres present in dense clusters.

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