Man man Lin, Bin Xu, huilu yao, Aiguo Shen and Jiming Hu
We report on a continuous and non-invasive approach in vivo to monitor arterial blood pH based on laser trapping and Raman detection of single live erythrocytes. A home-built confocal laser tweezers Raman system (LTRS) is applied to trace the live erythrocytes under different pH values of extracellular environment to record their corresponding Raman changes in vitro and in vivo. The analysis results in vitro show that when the extracellular environment pH changes from 6.5 to 9.0, two Raman intensity ratio (R1603, 1616=I1603/I1616) of single erythrocytes decreases regularly, what is more, there has a good linear relationship between these two variables, and the linearity is 0.985, which is also verified successfully via in vivo Raman measurements. These results demonstrate that the Raman signal of single live erythrocytes is possible as a marker of extracellular pH value. This in vivo and quantitative Raman-pH sensor of arterial blood will be an important candidate for monitoring the acid-base status during treatment of ill patients and some major surgery because of its continuous and non-invasive characters.
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