Use of Raman optical tweezers for cell cycle analysis

Sunita Ahlawat, Aniket Chowdhury, Abha Uppal, Nitin Kumar and Pradeep Kumar Gupta

We report the results of our investigations on the use of Raman optical tweezers for label free analysis of cells in different phases of their cell cycle. The studies performed on human colon adenocarcinoma (Colo-205) cells synchronized in G0/G1 and G2/M phases showed that the DNA Raman band at 783 cm-1 in the Raman spectra of optically trapped cells can provide information about the DNA content in the nucleus of the cell without the need for isolation of nucleus. The histograms of intensity of this band among the cell populations were found to corroborate the results obtained from fluorescence image cytometry performed on DAPI stained cells.

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