We developed a simple method to investigate the motion of a small particle in biopolymer solutions. Using optical tweezers with low stiffness, a trapped probe particle fluctuates widely for a long time along the light axis, which reflects the rheological properties of the surrounding environment. We present a convenient technique for three-dimensional position tracking and the analysis focused on the distribution of particle positions and its variance in a given time interval. It allows us to obtain useful information about the dynamics of a small particle in a wide range from a free diffusive motion to a constrained motion with statistical significance. We applied this method to investigate the dynamics in collagen and DNA solutions; it was found that a collagen solution behaves as a simple viscous liquid and a DNA solution has apparent elasticity due to the slow relaxation of the configuration of molecules.
DOI
DOI
No comments:
Post a Comment