Yoshio Tanaka and Shin-ich Wakida
Controlled three-dimensional (3D) rotation of arbitrarily shaped objects in the observation space of optical microscopes is essential for realizing tomographic microscope imaging and offers great flexibility as a noncontact micromanipulation tool for biomedical applications. Herein, we present 3D rotational control of inhomogeneous biological samples using 3D optical multiple-force clamps based on time-shared scanning with a fast focus-tunable lens. For inhomogeneous samples with shape and optical anisotropy, we choose diatoms and their fragments, and demonstrate interactive and controlled 3D rotation about arbitrary axes in 3D Cartesian coordinates. We also outline the hardware setup and 3D rotation method for our demonstrations.
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