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Saturday, June 29, 2013

Rigid DNA Beams for High-Resolution Single-Molecule Mechanics

Emanuel Pfitzner, Christian Wachauf, Fabian Kilchherr, Benjamin Pelz, William M. Shih, Matthias Rief, Prof. Dr. Hendrik Dietz
Bridging the gap: Rigid DNA linkers (blue, see picture) between microspheres (green) for high-resolution single-molecule mechanical experiments were constructed using DNA origami. The resulting DNA helical bundles greatly reduce the noise generated in studies of conformation changes using optical tweezers and were applied to study small DNA secondary structures.
DOI

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