Anna-Karin Gustavsson, David D. van Niekerk, Caroline B. Adiels, Franco du Preez, Mattias Goksor, Jacky L. Snoep
Yeast glycolytic oscillations have been studied since the 1950s in cell free extracts and in intact cells. Thus far, for intact cells sustained oscillations have only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microuidic chamber, we were able to observe sustained oscillations in individual, isolated cells. With a detailed kinetic model for the cellular reactions we could simulate the heterogeneity in the response of the individual cells assuming small diferences in a single internal parameter. This is the first time that sustained limit cycle oscillations are shown in isolated yeast cells.
DOI
Yeast glycolytic oscillations have been studied since the 1950s in cell free extracts and in intact cells. Thus far, for intact cells sustained oscillations have only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microuidic chamber, we were able to observe sustained oscillations in individual, isolated cells. With a detailed kinetic model for the cellular reactions we could simulate the heterogeneity in the response of the individual cells assuming small diferences in a single internal parameter. This is the first time that sustained limit cycle oscillations are shown in isolated yeast cells.
DOI
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