Single-Molecule DNA Force Spectroscopy to Probe Interactions with the Tri-Peptide Lys-Trp-Lys

Dr. Chandrashekhar U. Murade, Prof. Dr. Vinod Subramaniam, Dr. Cees Otto, Dr. Martin L. Bennink

May the force be with you: It is shown that single-molecule optical tweezers force spectroscopy can be used successfully to detect the effects of binding of low-affinity ligands to a single DNA molecule (see picture) and to determine various kinetic parameters of the reaction at single-molecular level.

DOI