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Monday, August 29, 2011

Individual particle handling in a microfluidic system based on parallel laser trapping

Philippe Hamel, Bastien Rachet, Michael Werner, Mathieu Grossenbacher, Horst Vogel, Martin Forrer, Peter Ryser, and René P. Salathé
We present an optical trapping system combining individually addressable multiple laser traps with fluorescence spectroscopy. An in-line set of 64 near-IR laser diodes is used to create a line of individually addressable traps inside a microfluidic chip. This system is completed by an excitation/detection line for spectrally resolved fluorescence imaging of trapped particles. Highly parallel trapping in a constant flow (up to a few millimeters per second), fast particle handling rates (up to a few particles per second), and the possibility of recording fluorescence spectra of trapped objects lead to a performing bioanalytical platform, e.g., for highly parallel analysis and sorting.

DOI

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