A dual optical tweezer for microrheology of bacterial suspensions

Yogesha , Raghu, A. , Nagesh, B.V. , Bhattacharya, S. , Mohana, D.C. , Ananthamurthy, S.

A dual optical tweezer has been built around an inverted microscope with high numerical aperture objective (N.A 1.4). The setup is versatile and can be used both as a single and a dual tweezer, and in the dual mode, enables us to optically trap two micron-sized latex beads within a few microns from each other in solution. Using this setup, we report measurements of the microrheological parameters of Pseudomonas fluorescens and Bacillus subtilis bacterial suspensions. We study the variation of viscoelastic moduli of these bacterial suspensions as a function of their cell count in solution. A comparison with inactive bacteria of corresponding cell count enables us to characterize the activity of the bacterial samples in terms of an average force that the bacteria exerts on the trapped bead. This work paves way for studies of interesting nonlinear rheological phenomena at small length scales.

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