Chie Hosokawa, Suguru N. Kudoh, Ai Kiyohara, and Takahisa Taguchi
We demonstrate intracellular manipulation of synaptic vesicles in living neurons by optical trapping. When an infrared trapping laser is focused on synapses of a neuronal cell labeled with a fluorescent endocytic marker, fluorescence is observed at the focal spot. The fluorescence spectrum is attributed to fluorescent dye in the synaptic vesicles, indicating excitation by two-photon absorption of the trapping laser. The fluorescence intensity increases gradually within ∼ 100 s of laser irradiation, suggesting that trapping force causes vesicles assembly at the focus. Our method can be applied to manipulate synaptic transmission of a particular neuron in a neuronal network.
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