Laser tweezers Raman spectrum of normal breast cell line and carcinoma breast cell line

Xie, Y.-A., Leng, Z.-H., Meng, L.-J. , Luo, X.-L. , Zhang, W.-M. , Kuang, Z.-P.

OBJECTIVE: To detect the interaction between Raman spectrum of normal breast cell line (HBL-100) and carcinoma breast cell line (MCF-7), as well as the change of biological tissue composition from normal cell to carcinogenesis. In the end, Characteristic Raman spectra of cancer cells were presented. METHODS: Raman spectrum of normal breast cell line and carcinoma breast cell line were recorded, The result of which was performed by principal component analysis(PCA). RESULTS: Significant differences of average Raman spectrums were founded between the normal cell and the carcinoma one; The spectra line of carcinoma cell became strong at the whole; The strength of 936, 1002, 1298 and 1445 cm-1 increased; the peak of 1102 cm-1 shifted to 1094 cm-1; the peak of 484 cm-1 disappeared; Additionally, the structure and the amount of protein, nucleic acid, lipids and other such molecules had also changed. Average Raman spectrum of monocell was analyzed with the principal component of PCA, the result of which suggested normal cell and carcinoma cell could be differentiated with PCA, The discrimination rate was 87% (13/15). CONCLUSIONS: Judging normal breast cell and carcinoma cell with laser tweezers Raman spectrums is an efficient method. Single cells laser tweezers raman spectra technique can become a new way to diagnose cancer, which has very wide prospect.

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