René Pflugradt, Ulrike Schmidt, Benjamin Landenberger, Timo Sänger and Sabine Lutz-Bonengel
To investigate the set of mtDNA molecules contained in small biological structures, powerful techniques for separation are required. We tested flow cytometry (FCM1), laser capture microdissection (LCM2) and a method using optical tweezers (OT3) in combination with a 1μ-Ibidi-Slide with regard to their ability to deposit single mitochondrial particles. The success of separation was determined by real-time quantitative PCR (qPCR4) and sequencing analysis.
OT revealed the highest potential for the separation and deposition of single mitochondrial particles. The study presents a novel setup for effective separation of single mitochondrial particles, which is crucial for the analysis of single mitochondria.
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