Holographic optical tweezers allow the creation of multiple optical traps in 3D configurations through the use of dynamic diffractive optical elements called spatial light modulators (SLMs). We show that, in addition to controlling traps, the SLM in a holographic tweezers system can be both the principal element of a wavefront sensor and the corrective element in a closed-loop adaptive optics system. This means that aberrations in such systems can be estimated and corrected without altering the experimental setup. Aberrations are estimated using the Shack–Hartmann method, where an array of spots is projected into the sample plane and the distortion of this array is used to recover the aberration. The system can recover aberrations of up to ten wavelengths peak–peak, and is sensitive to aberrations much smaller than a wavelength. The spot pattern could also be analysed by eye, as a tool for aligning the system.
DOI
DOI
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