Holographic optical tweezers typically require microscope objectives with high numerical aperture and thus usually suffer from the disadvantage of a small field of view and a small working distance. We experimentally investigate an optical mirror trap that is created after reflection of two holographically shaped collinear beams on a mirror. This approach combines a large field of view and a large working distance with the possibility to manipulate particles in a large size range, since it allows to use a microscope objective with a numerical aperture as low as 0.2. In this work we demonstrate robust optical three-dimensional trapping in a range of 1mm x 1mm x 2mm with particle sizes ranging from 1.4 μm up to 45 μm. The use of spatial light modulator based holographic methods to create the trapping beams allows to simultaneously trap many beads in complex, dynamic configurations. We present measurements that characterize the mirror traps in terms of trap stiffness, maximum trapping force and capture range.
Tuesday, October 13, 2009
Optical mirror trap with a large field of view
Maximilian Pitzek, Ruth Steiger, Gregor Thalhammer, Stefan Bernet, and Monika Ritsch-Marte
Holographic optical tweezers typically require microscope objectives with high numerical aperture and thus usually suffer from the disadvantage of a small field of view and a small working distance. We experimentally investigate an optical mirror trap that is created after reflection of two holographically shaped collinear beams on a mirror. This approach combines a large field of view and a large working distance with the possibility to manipulate particles in a large size range, since it allows to use a microscope objective with a numerical aperture as low as 0.2. In this work we demonstrate robust optical three-dimensional trapping in a range of 1mm x 1mm x 2mm with particle sizes ranging from 1.4 μm up to 45 μm. The use of spatial light modulator based holographic methods to create the trapping beams allows to simultaneously trap many beads in complex, dynamic configurations. We present measurements that characterize the mirror traps in terms of trap stiffness, maximum trapping force and capture range.
Holographic optical tweezers typically require microscope objectives with high numerical aperture and thus usually suffer from the disadvantage of a small field of view and a small working distance. We experimentally investigate an optical mirror trap that is created after reflection of two holographically shaped collinear beams on a mirror. This approach combines a large field of view and a large working distance with the possibility to manipulate particles in a large size range, since it allows to use a microscope objective with a numerical aperture as low as 0.2. In this work we demonstrate robust optical three-dimensional trapping in a range of 1mm x 1mm x 2mm with particle sizes ranging from 1.4 μm up to 45 μm. The use of spatial light modulator based holographic methods to create the trapping beams allows to simultaneously trap many beads in complex, dynamic configurations. We present measurements that characterize the mirror traps in terms of trap stiffness, maximum trapping force and capture range.
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