A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our method combines a liquid flow cell, fluorescence microscopy, and an optical-trap to facilitate simple measurements of the shear modulus and buckling properties of single RBCs, under physiological conditions. The efficacy of the method is illustrated by studying buckling behavior ofnormal and Plasmodium-infected RBCs, and the effect of Plasmodium falciparum–conditioned medium on normal, uninfected cells. Our simple method, which quantifiessingle-RBC deformability, may ease detection of RBC hematological disorders.
Wednesday, March 4, 2009
Measuring erythrocyte deformability with fluorescence, fluid forces, and optical trapping
Bambardekar, K, Dharmadhikari, AK, Dharmadhikari, JA , Mathur, D, Sharma, S
A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our method combines a liquid flow cell, fluorescence microscopy, and an optical-trap to facilitate simple measurements of the shear modulus and buckling properties of single RBCs, under physiological conditions. The efficacy of the method is illustrated by studying buckling behavior ofnormal and Plasmodium-infected RBCs, and the effect of Plasmodium falciparum–conditioned medium on normal, uninfected cells. Our simple method, which quantifiessingle-RBC deformability, may ease detection of RBC hematological disorders.
A laser-based method has been developed for experimentally probing single red blood cell (RBC) buckling and determining RBC membrane rigidity. Our method combines a liquid flow cell, fluorescence microscopy, and an optical-trap to facilitate simple measurements of the shear modulus and buckling properties of single RBCs, under physiological conditions. The efficacy of the method is illustrated by studying buckling behavior ofnormal and Plasmodium-infected RBCs, and the effect of Plasmodium falciparum–conditioned medium on normal, uninfected cells. Our simple method, which quantifiessingle-RBC deformability, may ease detection of RBC hematological disorders.
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