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Thursday, March 19, 2009

Flagella-generated forces reveal gear-type motor in single cells of the green alga, Chlamydomonas reinhardtii

Jacinta S. D’Souza, Mohanram Gudipati, Jayashree A. Dharmadhikari, Aditya K. Dharmadhikari, Abhishek Kashyap, Manaswini Aiyer, Usha Rao, Deepak Mathur and Basuthkar J. Rao

Optically trapped single cells of the biflagellated, green alga, Chlamydomonas reinhardtii, rotate. The rotational dynamics of trapped wild-type and mutant cells show that functional flagella play a decisive role: the entire flagellar apparatus (central microtubules, radial spokes, and dynein arms) is involved. Any aberration in this apparatus leads to non-functionality, indicating a gear-type mechanism. The translational and rotational motions of the wild-type and mutant cells do not differ significantly. Optical forces alone do not play a vital role in the rotational dynamics of this cellular motor, making them useful as probes of the internal dynamics without external influence.

DOI

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