R M Abraham Ekeroth
When illuminated by light, optically coupled nanoparticles suffer the action of multiple electromagnetic forces. In general, two kinds of forces are assumed: binding forces that make the particles attract/repel each other and scattering forces that push the system forwards. Tangential forces and orbital torques can also be induced to align the interacting particles with the electric field. In this work, new degrees of freedom were found for two coupled silica nanospheres under illumination with linearly-polarized plane waves. The results have a general validity for arbitrary mesoscale systems: multiple scattering of light induces unusual torques and deviation forces. These torques include spin contributions to the movement of the whole system. The results are supported by previous works and pave the way for the engineering of nanoscale devices and nanorotators. Any application based on photonics at mesoscales should take into account the new movements predicted here.
DOI
Wednesday, March 27, 2019
Is it possible to enlarge the trapping range of optical tweezers via a single beam?
X. Z. Li, H. X. Ma, H. Zhang, M. M. Tang, H. H. Li, J. Tang, and Y. S. Wang
For optical tweezers, a tiny focal spot of the trapping beam is necessary for providing sufficient intensity-gradient force. This condition results in a limited small trapping range to guarantee stable trapping of the particle. Exploiting structured light, i.e., an optical vortex beam, the trapping range can be enlarged by adjusting its doughnut ring diameter. However, the trapped particle scarcely remains static due to the optical spanner action of the orbital angular momentum of the vortex beam. To enlarge the trapping range and simultaneously ensure stable trapping, we propose a beam, referred to as a mirror-symmetric optical vortex beam (MOV). Essentially, MOV is constructed by using two opposite optical spanners and a pair of static optical tweezers. The optical spanners attract the particle to the site of the static optical tweezers, which realizes long-range optical trapping. Through detailed force-field analysis, it is found that MOV could perform these setting functions. In experiments, yeast cells are manipulated in a long range of ∼25 μm, which is 3 times longer than that of the Gaussian beam. Further, the trapping range is easily adjusted by changing a parameter as desired. This technique provides versatile optical tweezers, which will facilitate potential applications for particle manipulation.
DOI
For optical tweezers, a tiny focal spot of the trapping beam is necessary for providing sufficient intensity-gradient force. This condition results in a limited small trapping range to guarantee stable trapping of the particle. Exploiting structured light, i.e., an optical vortex beam, the trapping range can be enlarged by adjusting its doughnut ring diameter. However, the trapped particle scarcely remains static due to the optical spanner action of the orbital angular momentum of the vortex beam. To enlarge the trapping range and simultaneously ensure stable trapping, we propose a beam, referred to as a mirror-symmetric optical vortex beam (MOV). Essentially, MOV is constructed by using two opposite optical spanners and a pair of static optical tweezers. The optical spanners attract the particle to the site of the static optical tweezers, which realizes long-range optical trapping. Through detailed force-field analysis, it is found that MOV could perform these setting functions. In experiments, yeast cells are manipulated in a long range of ∼25 μm, which is 3 times longer than that of the Gaussian beam. Further, the trapping range is easily adjusted by changing a parameter as desired. This technique provides versatile optical tweezers, which will facilitate potential applications for particle manipulation.
DOI
DNA Intercalation Facilitates Efficient DNA-Targeted Covalent Binding of Phenanthriplatin
Ali A. Almaqwashi, Wen Zhou, M. Nabuan Naufer, Imogen A. Ridde, Ömer H. Yilmaz, Stephen J. Lippard, and Mark C. Williams
Phenanthriplatin, a monofunctional anticancer agent derived from cisplatin, shows significantly more rapid DNA covalent-binding activity compared to its parent complex. To understand the underlying molecular mechanism, we used single-molecule studies with optical tweezers to probe the kinetics of DNA–phenanthriplatin binding as well as DNA binding to several control complexes. The time-dependent extensions of single λ-DNA molecules were monitored at constant applied forces and compound concentrations, followed by rinsing with a compound-free solution. DNA–phenanthriplatin association consisted of fast and reversible DNA lengthening with time constant τ ≈ 10 s, followed by slow and irreversible DNA elongation that reached equilibrium in ∼30 min. In contrast, only reversible fast DNA elongation occured for its stereoisomer trans-phenanthriplatin, suggesting that the distinct two-rate kinetics of phenanthriplatin is sensitive to the geometric conformation of the complex. Furthermore, no DNA unwinding was observed for pyriplatin, in which the phenanthridine ligand of phenanthriplatin is replaced by the smaller pyridine molecule, indicating that the size of the aromatic group is responsible for the rapid DNA elongation. These findings suggest that the mechanism of binding of phenanthriplatin to DNA involves rapid, partial intercalation of the phenanthridine ring followed by slower substitution of the adjacent chloride ligand by, most likely, the N7 atom of a purine base. The cis isomer affords the proper stereochemistry at the metal center to facilitate essentially irreversible DNA covalent binding, a geometric advantage not afforded by trans-phenanthriplatin. This study demonstrates that reversible DNA intercalation provides a robust transition state that is efficiently converted to an irreversible DNA-Pt bound state.
DOI
Phenanthriplatin, a monofunctional anticancer agent derived from cisplatin, shows significantly more rapid DNA covalent-binding activity compared to its parent complex. To understand the underlying molecular mechanism, we used single-molecule studies with optical tweezers to probe the kinetics of DNA–phenanthriplatin binding as well as DNA binding to several control complexes. The time-dependent extensions of single λ-DNA molecules were monitored at constant applied forces and compound concentrations, followed by rinsing with a compound-free solution. DNA–phenanthriplatin association consisted of fast and reversible DNA lengthening with time constant τ ≈ 10 s, followed by slow and irreversible DNA elongation that reached equilibrium in ∼30 min. In contrast, only reversible fast DNA elongation occured for its stereoisomer trans-phenanthriplatin, suggesting that the distinct two-rate kinetics of phenanthriplatin is sensitive to the geometric conformation of the complex. Furthermore, no DNA unwinding was observed for pyriplatin, in which the phenanthridine ligand of phenanthriplatin is replaced by the smaller pyridine molecule, indicating that the size of the aromatic group is responsible for the rapid DNA elongation. These findings suggest that the mechanism of binding of phenanthriplatin to DNA involves rapid, partial intercalation of the phenanthridine ring followed by slower substitution of the adjacent chloride ligand by, most likely, the N7 atom of a purine base. The cis isomer affords the proper stereochemistry at the metal center to facilitate essentially irreversible DNA covalent binding, a geometric advantage not afforded by trans-phenanthriplatin. This study demonstrates that reversible DNA intercalation provides a robust transition state that is efficiently converted to an irreversible DNA-Pt bound state.
DOI
Comparison of single-neutral-atom qubit between in bright trap and in dark trap
Ya-Li Tian, Zhi-Hui Wang, Peng-Fei Yang, Peng-Fei Zhang, Gang Li and Tian-Cai Zhang
A single neutral atom is one of the most promising candidates to encode a quantum bit (qubit). In a real experiment, a single neutral atom is always confined in a micro-sized far off-resonant optical trap (FORT). There are generally two types of traps: red-detuned trap and blue-detuned trap. We experimentally compare the qubits encoded in "clock states" of single cesium atoms confined separately in either 1064-nm red-detuned (bright) trap or 780-nm blue-detuned (dark) trap: both traps have almost the same trap depth. A longer lifetime of 117 s and a longer coherence time of about 10 ms are achieved in the dark trap. This provides a direct proof of the superiority of the dark trap over the bright trap. The measures to further improve the coherence are discussed.
DOI
A single neutral atom is one of the most promising candidates to encode a quantum bit (qubit). In a real experiment, a single neutral atom is always confined in a micro-sized far off-resonant optical trap (FORT). There are generally two types of traps: red-detuned trap and blue-detuned trap. We experimentally compare the qubits encoded in "clock states" of single cesium atoms confined separately in either 1064-nm red-detuned (bright) trap or 780-nm blue-detuned (dark) trap: both traps have almost the same trap depth. A longer lifetime of 117 s and a longer coherence time of about 10 ms are achieved in the dark trap. This provides a direct proof of the superiority of the dark trap over the bright trap. The measures to further improve the coherence are discussed.
DOI
Friday, March 15, 2019
Numerical simulation of optical control for a soft particle in a microchannel
Ji Young Moon, Se Bin Choi, Jung Shin Lee, Roger I. Tanner, and Joon Sang Lee
Technologies that use optical force to actively control particles in microchannels are a significant area of research interest in various fields. An optical force is generated by the momentum change caused by the refraction and reflection of light, which changes the particle surface as a function of the angle of incidence of light and which in turn feeds back and modifies the force on the particle. Simulating this phenomenon is a complex task. The deformation of a particle, the interaction between the surrounding fluid and the particle, and the reflection and refraction of light should be analyzed simultaneously. Herein, a deformable particle in a microchannel subjected to optical interactions is simulated using the three-dimensional lattice Boltzmann immersed-boundary method. The laser from the optical source is analyzed by dividing it into individual rays. To calculate the optical forces exerted on the particle, the intensity, momentum, and ray direction are calculated. The optical-separator problem with one optical source is analyzed by measuring the distance traveled because of the optical force. The optical-stretcher problem with two optical sources is then studied by analyzing the relation between the intensity of the optical source and particle deformation. This simulation will help the design of sorting and measuring by optical force.
DOI
Technologies that use optical force to actively control particles in microchannels are a significant area of research interest in various fields. An optical force is generated by the momentum change caused by the refraction and reflection of light, which changes the particle surface as a function of the angle of incidence of light and which in turn feeds back and modifies the force on the particle. Simulating this phenomenon is a complex task. The deformation of a particle, the interaction between the surrounding fluid and the particle, and the reflection and refraction of light should be analyzed simultaneously. Herein, a deformable particle in a microchannel subjected to optical interactions is simulated using the three-dimensional lattice Boltzmann immersed-boundary method. The laser from the optical source is analyzed by dividing it into individual rays. To calculate the optical forces exerted on the particle, the intensity, momentum, and ray direction are calculated. The optical-separator problem with one optical source is analyzed by measuring the distance traveled because of the optical force. The optical-stretcher problem with two optical sources is then studied by analyzing the relation between the intensity of the optical source and particle deformation. This simulation will help the design of sorting and measuring by optical force.
DOI
DNA stretching induces Cas9 off-target activity
Matthew D. Newton, Benjamin J. Taylor, Rosalie P. C. Driessen, Leonie Roos, Nevena Cvetesic, Shenaz Allyjaun, Boris Lenhard, Maria Emanuela Cuomo & David S. Rueda
CRISPR/Cas9 is a powerful genome-editing tool, but spurious off-target edits present a barrier to therapeutic applications. To understand how CRISPR/Cas9 discriminates between on-targets and off-targets, we have developed a single-molecule assay combining optical tweezers with fluorescence to monitor binding to λ-DNA. At low forces, the Streptococcus pyogenes Cas9 complex binds and cleaves DNA specifically. At higher forces, numerous off-target binding events appear repeatedly at the same off-target sites in a guide-RNA-sequence-dependent manner, driven by the mechanical distortion of the DNA. Using single-molecule Förster resonance energy transfer (smFRET) and cleavage assays, we show that DNA bubbles induce off-target binding and cleavage at these sites, even with ten mismatches, as well as at previously identified in vivo off-targets. We propose that duplex DNA destabilization during cellular processes (for example, transcription, replication, etc.) can expose these cryptic off-target sites to Cas9 activity, highlighting the need for improved off-target prediction algorithms.
DOI
CRISPR/Cas9 is a powerful genome-editing tool, but spurious off-target edits present a barrier to therapeutic applications. To understand how CRISPR/Cas9 discriminates between on-targets and off-targets, we have developed a single-molecule assay combining optical tweezers with fluorescence to monitor binding to λ-DNA. At low forces, the Streptococcus pyogenes Cas9 complex binds and cleaves DNA specifically. At higher forces, numerous off-target binding events appear repeatedly at the same off-target sites in a guide-RNA-sequence-dependent manner, driven by the mechanical distortion of the DNA. Using single-molecule Förster resonance energy transfer (smFRET) and cleavage assays, we show that DNA bubbles induce off-target binding and cleavage at these sites, even with ten mismatches, as well as at previously identified in vivo off-targets. We propose that duplex DNA destabilization during cellular processes (for example, transcription, replication, etc.) can expose these cryptic off-target sites to Cas9 activity, highlighting the need for improved off-target prediction algorithms.
DOI
Modal approach to optical forces between waveguides as derived by transformation optics formalism
Hideo Iizuka and Shanhui Fan
Optical force between two lossless waveguides has been described by two approaches. One approach is the explicit description of the force by the Maxwell stress tensor. Another approach is to describe the modal force in terms of the derivative of the eigenmode frequency with respect to the distance variation. Here, we analytically prove the equivalence of these two approaches for lossless waveguides having arbitrary cross sections through the use of transformation optics formalism. Our derivation is applicable to both pressure and shear forces. We also show that these two approaches are not equivalent in the presence of loss.
DOI
Optical force between two lossless waveguides has been described by two approaches. One approach is the explicit description of the force by the Maxwell stress tensor. Another approach is to describe the modal force in terms of the derivative of the eigenmode frequency with respect to the distance variation. Here, we analytically prove the equivalence of these two approaches for lossless waveguides having arbitrary cross sections through the use of transformation optics formalism. Our derivation is applicable to both pressure and shear forces. We also show that these two approaches are not equivalent in the presence of loss.
DOI
Efficient optical trapping with cylindrical vector beams
H. Moradi, V. Shahabadi, E. Madadi, E. Karimi, and F. Hajizadeh
Radially and azimuthally polarized beams can create needle-like electric and magnetic fields under tight focusing conditions, respectively, and thus have been highly recommended for optical manipulation. There have been reports on the superiority of these beams over the conventional Gaussian beam for providing a larger optical force in single beam optical trap. However, serious discrepancies in their experimental results prevent one from concluding this superiority. Here, we theoretically and experimentally study the impact of different parameters — such as spherical aberration, the numerical aperture of the focusing lens, and the particles’ size — on optical trapping stiffness of radially, azimuthally, and linearly polarized beams. The result of calculations based on generalized Lorenz–Mie theory, which is in good agreement with the experiment, reveals that the studied parameters determine which polarization state has the superiority for optical trapping. Our findings play a crucial role in the development of optical tweezers setups and, in particular, in biophysical applications when laser-induced heating in the optical tweezers applications is the main concern.
DOI
Radially and azimuthally polarized beams can create needle-like electric and magnetic fields under tight focusing conditions, respectively, and thus have been highly recommended for optical manipulation. There have been reports on the superiority of these beams over the conventional Gaussian beam for providing a larger optical force in single beam optical trap. However, serious discrepancies in their experimental results prevent one from concluding this superiority. Here, we theoretically and experimentally study the impact of different parameters — such as spherical aberration, the numerical aperture of the focusing lens, and the particles’ size — on optical trapping stiffness of radially, azimuthally, and linearly polarized beams. The result of calculations based on generalized Lorenz–Mie theory, which is in good agreement with the experiment, reveals that the studied parameters determine which polarization state has the superiority for optical trapping. Our findings play a crucial role in the development of optical tweezers setups and, in particular, in biophysical applications when laser-induced heating in the optical tweezers applications is the main concern.
DOI
Plasmonic field guided patterning of ordered colloidal nanostructures
Xiaoping Huang, Kai Chen, Mingxi Qi, Peifeng Zhang, Yu Li, Stephan Winnerl, Harald Schneider, Yuanjie Yang, Shuang Zhang
Nano-patterned colloidal plasmonic metasurfaces are capable of manipulation of light at the subwavelength scale. However, achieving controllable lithography-free nano-patterning for colloidal metasurfaces still remains a major challenge, limiting their full potential in building advanced plasmonic devices. Here, we demonstrate plasmonic field guided patterning (PFGP) of ordered colloidal metallic nano-patterns using orthogonal laser standing evanescent wave (LSEW) fields. We achieved colloidal silver nano-patterns with a large area of 30 mm2 in <10 min by using orthogonal LSEW fields with a non-focused ultralow fluence irradiation of 0.25 W cm−2. The underlying mechanism of the formation of the nano-patterns is the light-induced polarization of the nanoparticles (NPs), which leads to a dipole-dipole interaction for stabilizing the nano-pattern formation, as confirmed by polarization-dependent surface-enhanced Raman spectroscopy. This optical field-directed self-assembly of NPs opens an avenue for designing and fabricating reconfigurable colloidal nano-patterned metasurfaces in large areas.
DOI
Nano-patterned colloidal plasmonic metasurfaces are capable of manipulation of light at the subwavelength scale. However, achieving controllable lithography-free nano-patterning for colloidal metasurfaces still remains a major challenge, limiting their full potential in building advanced plasmonic devices. Here, we demonstrate plasmonic field guided patterning (PFGP) of ordered colloidal metallic nano-patterns using orthogonal laser standing evanescent wave (LSEW) fields. We achieved colloidal silver nano-patterns with a large area of 30 mm2 in <10 min by using orthogonal LSEW fields with a non-focused ultralow fluence irradiation of 0.25 W cm−2. The underlying mechanism of the formation of the nano-patterns is the light-induced polarization of the nanoparticles (NPs), which leads to a dipole-dipole interaction for stabilizing the nano-pattern formation, as confirmed by polarization-dependent surface-enhanced Raman spectroscopy. This optical field-directed self-assembly of NPs opens an avenue for designing and fabricating reconfigurable colloidal nano-patterned metasurfaces in large areas.
DOI
Reactive optical matter: light-induced motility in electrodynamically asymmetric nanoscale scatterers
Yuval Yifat, Delphine Coursault, Curtis W. Peterson, John Parker, Ying Bao, Stephen K. Gray, Stuart A. Rice & Norbert F. Scherer
From Newton’s third law, which is known as the principle of actio et reactio1, we expect the forces between interacting particles to be equal and opposite for closed systems. Otherwise, “nonreciprocal” forces can arise.2 This has been shown theoretically in the interaction between dissimilar optically trapped particles that are mediated by an external field.3 As a result, despite the incident external field not having a transverse component of momentum, the particle pair experiences a force in a direction that is transverse to the light propagation direction.3,4 In this letter, we directly measure the net nonreciprocal forces in electrodynamically interacting asymmetric nanoparticle dimers and nanoparticle structures that are illuminated by plane waves and confined to pseudo one-dimensional geometries. We show via electrodynamic theory and simulations that interparticle interactions cause asymmetric scattering from heterodimers. Therefore, the putative nonreciprocal forces are actually a consequence of momentum conservation. Our study demonstrates that asymmetric scatterers exhibit directed motion due to the breakdown of mirror symmetry in their electrodynamic interactions with external fields.
From Newton’s third law, which is known as the principle of actio et reactio1, we expect the forces between interacting particles to be equal and opposite for closed systems. Otherwise, “nonreciprocal” forces can arise.2 This has been shown theoretically in the interaction between dissimilar optically trapped particles that are mediated by an external field.3 As a result, despite the incident external field not having a transverse component of momentum, the particle pair experiences a force in a direction that is transverse to the light propagation direction.3,4 In this letter, we directly measure the net nonreciprocal forces in electrodynamically interacting asymmetric nanoparticle dimers and nanoparticle structures that are illuminated by plane waves and confined to pseudo one-dimensional geometries. We show via electrodynamic theory and simulations that interparticle interactions cause asymmetric scattering from heterodimers. Therefore, the putative nonreciprocal forces are actually a consequence of momentum conservation. Our study demonstrates that asymmetric scatterers exhibit directed motion due to the breakdown of mirror symmetry in their electrodynamic interactions with external fields.
Wednesday, March 6, 2019
Measuring local properties inside a cell‐mimicking structure using rotating optical tweezers
Shu Zhang Lachlan J. Gibson Alexander B. Stilgoe Timo A. Nieminen Halina Rubinsztein‐Dunlop
Exploring the rheological properties of intracellular materials is essential for understanding cellular and subcellular processes. Optical traps have been widely used for physical manipulation of micro‐ and nano‐objects within fluids enabling studies of biological systems. However, experiments remain challenging as it is unclear how the probe particle’s mobility is influenced by the nearby membranes and organelles. We use liposomes (unilamellar lipid vesicles) as a simple biomimetic model of living cells, together with a trapped particle rotated by optical tweezers to study mechanical and rheological properties inside a liposome both theoretically and experimentally. Here we demonstrate that this system has the capacity to predict the hydrodynamic interaction between three‐dimensional (3D) spatial membranes and internal probe particles within submicron distances, and it has the potential to aid in the design of high resolution optical micro/nanorheology techniques to be used inside living cells.
DOI
Exploring the rheological properties of intracellular materials is essential for understanding cellular and subcellular processes. Optical traps have been widely used for physical manipulation of micro‐ and nano‐objects within fluids enabling studies of biological systems. However, experiments remain challenging as it is unclear how the probe particle’s mobility is influenced by the nearby membranes and organelles. We use liposomes (unilamellar lipid vesicles) as a simple biomimetic model of living cells, together with a trapped particle rotated by optical tweezers to study mechanical and rheological properties inside a liposome both theoretically and experimentally. Here we demonstrate that this system has the capacity to predict the hydrodynamic interaction between three‐dimensional (3D) spatial membranes and internal probe particles within submicron distances, and it has the potential to aid in the design of high resolution optical micro/nanorheology techniques to be used inside living cells.
DOI
Tunable Soft-Matter Optofluidic Waveguides Assembled by Light
Oto Brzobohatý, Lukáš Chvátal, Alexandr Jonáš, Martin Šiler, Jan Kaňka, Jan Ježek, and Pavel Zemánek
Development of artificial materials exhibiting unusual optical properties is one of the major strands of current photonics research. Of particular interest are soft-matter systems reconfigurable by external stimuli that play an important role in research fields ranging from physics to chemistry and life sciences. Here, we prepare and study unconventional self-assembled colloidal optical waveguides (CWs) created from wavelength-size dielectric particles held together by long-range optical forces. We demonstrate robust nonlinear optical properties of these CWs that lead to optical transformation characteristics remarkably similar to those of gradient refractive index materials and enable reversible all-optical tuning of light propagation through the CW. Moreover, we characterize strong optomechanical interactions responsible for the CW self-assembly; in particular, we report self-sustained oscillations of the whole CW structure tuned so that the wavelength of the laser beams forming the CW is not allowed to propagate through. The observed significant coupling between the mechanical motion of the CW and the intensity of light transmitted through the CW can form a base for designing novel mesoscopic-scale photonic devices that are reconfigurable by light.
DOIACS
Development of artificial materials exhibiting unusual optical properties is one of the major strands of current photonics research. Of particular interest are soft-matter systems reconfigurable by external stimuli that play an important role in research fields ranging from physics to chemistry and life sciences. Here, we prepare and study unconventional self-assembled colloidal optical waveguides (CWs) created from wavelength-size dielectric particles held together by long-range optical forces. We demonstrate robust nonlinear optical properties of these CWs that lead to optical transformation characteristics remarkably similar to those of gradient refractive index materials and enable reversible all-optical tuning of light propagation through the CW. Moreover, we characterize strong optomechanical interactions responsible for the CW self-assembly; in particular, we report self-sustained oscillations of the whole CW structure tuned so that the wavelength of the laser beams forming the CW is not allowed to propagate through. The observed significant coupling between the mechanical motion of the CW and the intensity of light transmitted through the CW can form a base for designing novel mesoscopic-scale photonic devices that are reconfigurable by light.
DOIACS
Additively manufacturable micro-mechanical logic gates
Yuanping Song, Robert M. Panas, Samira Chizari, Lucas A. Shaw, Julie A. Jackson, Jonathan B. Hopkins & Andrew J. Pascall
Early examples of computers were almost exclusively based on mechanical devices. Although electronic computers became dominant in the past 60 years, recent advancements in three-dimensional micro-additive manufacturing technology provide new fabrication techniques for complex microstructures which have rekindled research interest in mechanical computations. Here we propose a new digital mechanical computation approach based on additively-manufacturable micro-mechanical logic gates. The proposed mechanical logic gates (i.e., NOT, AND, OR, NAND, and NOR gates) utilize multi-stable micro-flexures that buckle to perform Boolean computations based purely on mechanical forces and displacements with no electronic components. A key benefit of the proposed approach is that such systems can be additively fabricated as embedded parts of microarchitected metamaterials that are capable of interacting mechanically with their surrounding environment while processing and storing digital data internally without requiring electric power.
DOI
Early examples of computers were almost exclusively based on mechanical devices. Although electronic computers became dominant in the past 60 years, recent advancements in three-dimensional micro-additive manufacturing technology provide new fabrication techniques for complex microstructures which have rekindled research interest in mechanical computations. Here we propose a new digital mechanical computation approach based on additively-manufacturable micro-mechanical logic gates. The proposed mechanical logic gates (i.e., NOT, AND, OR, NAND, and NOR gates) utilize multi-stable micro-flexures that buckle to perform Boolean computations based purely on mechanical forces and displacements with no electronic components. A key benefit of the proposed approach is that such systems can be additively fabricated as embedded parts of microarchitected metamaterials that are capable of interacting mechanically with their surrounding environment while processing and storing digital data internally without requiring electric power.
DOI
Manipulation and Deposition of Complex, Functional Block Copolymer Nanostructures using Optical Tweezers
Oliver E. C. Gould, Stuart J. Box, Charlotte E. Boott, Andrew D. Ward, Mitchell A. Winnik, Mervyn J. Miles, and Ian Manners
Block copolymer self-assembly has enabled the creation of a range of solution-phase nanostructures with applications from optoelectronics and biomedicine to catalysis. However, to incorporate such materials into devices a method that facilitates their precise manipulation and deposition is desirable. Herein we describe how optical tweezers can be used to trap, manipulate, and pattern individual cylindrical micelles and larger hybrid micellar materials. Through the combination of TIRF imaging and optical trapping we can precisely control the three-dimensional motion of individual cylindrical block copolymer micelles in solution, enabling the creation of customizable arrays. We also demonstrate that dynamic holographic assembly enables the creation of ordered customizable arrays of complex hybrid block copolymer structures. By creating a program which automatically identifies, traps and then deposits multiple assemblies simultaneously we have been able to dramatically speed up this normally slow process, enabling the fabrication of arrays of hybrid structures containing hundreds of assemblies in minutes rather than hours.
DOI
Block copolymer self-assembly has enabled the creation of a range of solution-phase nanostructures with applications from optoelectronics and biomedicine to catalysis. However, to incorporate such materials into devices a method that facilitates their precise manipulation and deposition is desirable. Herein we describe how optical tweezers can be used to trap, manipulate, and pattern individual cylindrical micelles and larger hybrid micellar materials. Through the combination of TIRF imaging and optical trapping we can precisely control the three-dimensional motion of individual cylindrical block copolymer micelles in solution, enabling the creation of customizable arrays. We also demonstrate that dynamic holographic assembly enables the creation of ordered customizable arrays of complex hybrid block copolymer structures. By creating a program which automatically identifies, traps and then deposits multiple assemblies simultaneously we have been able to dramatically speed up this normally slow process, enabling the fabrication of arrays of hybrid structures containing hundreds of assemblies in minutes rather than hours.
DOI
Colloidal Rare Earth Vanadate Single Crystalline Particles as Ratiometric Luminescent Thermometers
Paulo C. de Sousa Filho, Juliette Alain, Godefroy Leménager, Eric Larquet, Jochen Fick, Osvaldo A. Serra, and Thierry Gacoin
Thulium/ytterbium-doped yttrium vanadate particles provide a ratiometric thermal response as both colloids and powders via downshift or upconversion emissions. Here, we synthesized yttrium vanadates by controlled colloidal conversion of hydroxycarbonate precursors. A protected annealing process yielded single crystalline and readily dispersible particles that were manipulated individually by optical tweezers in water. Because individual particles displayed detectable emissions, this system has potential applications as a single-particle luminescent temperature sensor. Excitation on Yb3+ sensitizers (λexc = 980 nm) or at vanadate groups (λexc = 300 nm) resulted in Tm3+ emissions that effectively correlated with the temperature of the sample from 288 to 473 K with high relative thermal sensitivity (0.8–2.2% K–1), one of the highest reported for vanadate nanocrystals so far. Different pairs of Tm3+ transitions afford a ratiometric thermal response, which fitted common sensing requirements such as large [3F2,3 → 3H6 (λ = 700 nm)/1G4 → 3H6 (λ = 475 nm)] or small [3F2,3 → 3H6 (λ = 700 nm)/1G4 → 3F4 (λ = 650 nm)] spectral gaps and emission wavelengths at the first near-infrared biological window [3F2,3 → 3H6 (λ = 700 nm)/3H4 → 3H6 (λ = 800 nm)]. Our findings open new perspectives for the use of luminescent nanothermometers with controllable spatial localization, which is a remarkably interesting prospect to investigate microscopically localized events related to changes in temperature.
Thulium/ytterbium-doped yttrium vanadate particles provide a ratiometric thermal response as both colloids and powders via downshift or upconversion emissions. Here, we synthesized yttrium vanadates by controlled colloidal conversion of hydroxycarbonate precursors. A protected annealing process yielded single crystalline and readily dispersible particles that were manipulated individually by optical tweezers in water. Because individual particles displayed detectable emissions, this system has potential applications as a single-particle luminescent temperature sensor. Excitation on Yb3+ sensitizers (λexc = 980 nm) or at vanadate groups (λexc = 300 nm) resulted in Tm3+ emissions that effectively correlated with the temperature of the sample from 288 to 473 K with high relative thermal sensitivity (0.8–2.2% K–1), one of the highest reported for vanadate nanocrystals so far. Different pairs of Tm3+ transitions afford a ratiometric thermal response, which fitted common sensing requirements such as large [3F2,3 → 3H6 (λ = 700 nm)/1G4 → 3H6 (λ = 475 nm)] or small [3F2,3 → 3H6 (λ = 700 nm)/1G4 → 3F4 (λ = 650 nm)] spectral gaps and emission wavelengths at the first near-infrared biological window [3F2,3 → 3H6 (λ = 700 nm)/3H4 → 3H6 (λ = 800 nm)]. Our findings open new perspectives for the use of luminescent nanothermometers with controllable spatial localization, which is a remarkably interesting prospect to investigate microscopically localized events related to changes in temperature.
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