Rafal Kasztelanic, Adam Filipkowski, Alicja Anuszkiewicz, Paulina Stafiej, Grzegorz Stepniewski, Dariusz Pysz, Konrad Krzyzak, Ryszard Stepien, Mariusz Klimczak & Ryszard Buczynski
We present both a theoretical and an experimental study of a novel compact lensed fiber system utilizing a nanostructured GRIN lens. The lens can be integrated with an optical fiber, which ensures a unique and efficient focusing in any high index medium, such as a liquid. We use the effective medium approach to design lenses with arbitrary refractive index. To fabricate lenses, we utilize a discrete array of nano-sized rods made of two types of glasses, and apply a standard stack-and-draw fiber drawing technology. The fabricated nanostructured GRIN lenses have a parabolic refractive index profile with a diameter of a standard fiber, very short working distances (55 µm in the air) and a high numerical aperture (NA = 0.16). As a proof-of-concept of the new micro-lensed fiber system, we demonstrate an experiment on optical trapping of micrometer-sized glass beads. We also show that our method is compatible with optical fiber technology and allows for any shape of the refractive index distribution in 2D. Thanks to that a new functionality could be achieved by replacing the GRIN lens with an axicon lens, vortex type elements, micro-lenses arrays or diffraction elements.
DOI
Monday, April 30, 2018
Measurement of the mechanical properties of single Synechocystis sp. strain PCC6803 cells in different osmotic concentrations using a robot-integrated microfluidic chip
Di Chang, Shinya Sakuma, Kota Kera, Nobuyuki Uozumi and Fumihito Arai
Synechocystis sp. strain PCC6803 (Synechocystis) is a model microorganism and its mechanosensitive (MS) channels play important roles in its osmoadaptation mechanism. When the osmotic concentration of the culture environment changes, the inner pressure of the cell also changes due to the transportation of water through ion channels. Because the tension in the cell membrane relates to the inner pressure, we expect that the response of the MS channels to an osmotic concentration change could be evaluated by measuring their mechanical properties. Here, we propose a system for the measurement of the mechanical properties of a single Synechocystis cell. We developed a robot-integrated microfluidic chip combined with optical tweezers. The chip has an external actuated pushing probe and a force sensor probe. A single cell was located between the tip of both probes using the optical tweezers and was then deformed using the probes. As a result, we could measure the force and deformation and compare the Young's moduli of two groups: a group of wild type cells and a group of mutant (genetically modified) cells with a defect in the MS channels, at three different osmotic concentrations. The results showed that the Young's modulus of each group changed according to the osmotic concentration, while changes in cell size were too small to be detected. These results confirmed that the proposed evaluation method provides an understanding of the physiological function of MS channels for keeping the cell integrity of microorganisms when the cells are exposed to different external osmotic changes.
DOI
Synechocystis sp. strain PCC6803 (Synechocystis) is a model microorganism and its mechanosensitive (MS) channels play important roles in its osmoadaptation mechanism. When the osmotic concentration of the culture environment changes, the inner pressure of the cell also changes due to the transportation of water through ion channels. Because the tension in the cell membrane relates to the inner pressure, we expect that the response of the MS channels to an osmotic concentration change could be evaluated by measuring their mechanical properties. Here, we propose a system for the measurement of the mechanical properties of a single Synechocystis cell. We developed a robot-integrated microfluidic chip combined with optical tweezers. The chip has an external actuated pushing probe and a force sensor probe. A single cell was located between the tip of both probes using the optical tweezers and was then deformed using the probes. As a result, we could measure the force and deformation and compare the Young's moduli of two groups: a group of wild type cells and a group of mutant (genetically modified) cells with a defect in the MS channels, at three different osmotic concentrations. The results showed that the Young's modulus of each group changed according to the osmotic concentration, while changes in cell size were too small to be detected. These results confirmed that the proposed evaluation method provides an understanding of the physiological function of MS channels for keeping the cell integrity of microorganisms when the cells are exposed to different external osmotic changes.
DOI
Conical Refraction Bottle Beams for Entrapment of Absorbing Droplets
Michael Esseling, Christina Alpmann, Jens Schnelle, Robert Meissner & Cornelia Denz
Conical refraction (CR) optical bottle beams for photophoretic trapping of airborne absorbing droplets are introduced and experimentally demonstrated. CR describes the circular split-up of unpolarised light propagating along an optical axis in a biaxial crystal. The diverging and converging cones lend themselves to the construction of optical bottle beams with flexible entry points. The interaction of single inkjet droplets with an open or partly open bottle beam is shown implementing high-speed video microscopy in a dual-view configuration. Perpendicular image planes are visualized on a single camera chip to characterize the integral three-dimensional movement dynamics of droplets. We demonstrate how a partly opened optical bottle transversely confines liquid objects. Furthermore we observe and analyse transverse oscillations of absorbing droplets as they hit the inner walls and simultaneously measure both transverse and axial velocity components.
DOI
Conical refraction (CR) optical bottle beams for photophoretic trapping of airborne absorbing droplets are introduced and experimentally demonstrated. CR describes the circular split-up of unpolarised light propagating along an optical axis in a biaxial crystal. The diverging and converging cones lend themselves to the construction of optical bottle beams with flexible entry points. The interaction of single inkjet droplets with an open or partly open bottle beam is shown implementing high-speed video microscopy in a dual-view configuration. Perpendicular image planes are visualized on a single camera chip to characterize the integral three-dimensional movement dynamics of droplets. We demonstrate how a partly opened optical bottle transversely confines liquid objects. Furthermore we observe and analyse transverse oscillations of absorbing droplets as they hit the inner walls and simultaneously measure both transverse and axial velocity components.
DOI
Microparticle manipulation using femtosecond photonic nanojet-assisted laser cavitation
Aleksander Shakhov, Artyom Astafiev, and Viktor Nadtochenko
We report the effect of laser cavitation in water initiated by femtosecond pulses confined into subwavelength volume of photonic nanojet of spherical microparticles. The effect of nanoscale optical breakdown was employed for controllable and nondestructive micromanipulation of silica microspheres. We combine this technique with optical trapping for cyclic particle movements and estimate a peak velocity and an acceleration acquired by microspheres propelled by nanojet cavitation. Our study provides a strategy for nondestructive optical micromanipulation, cavitation-assisted drug delivery, and laser energy transduction in microdevices.
DOI
We report the effect of laser cavitation in water initiated by femtosecond pulses confined into subwavelength volume of photonic nanojet of spherical microparticles. The effect of nanoscale optical breakdown was employed for controllable and nondestructive micromanipulation of silica microspheres. We combine this technique with optical trapping for cyclic particle movements and estimate a peak velocity and an acceleration acquired by microspheres propelled by nanojet cavitation. Our study provides a strategy for nondestructive optical micromanipulation, cavitation-assisted drug delivery, and laser energy transduction in microdevices.
DOI
Evaluation of mitochondrial activity by two-photon absorption with near-field multioptical fiber probes
Yasuaki Kanazashi, Naoshi Takara, Kentaro Iwami, Yoshihiro Ohta and Norihiro Umeda
pH measurements enable the direct monitoring and evaluation of mitochondrial activity. We constructed a scanning near-field optical microscopy system with multioptical fiber probes using the two-photon absorption of a pH-sensitive fluorescent dye, SNARF-4F, to measure the activity difference of mitochondrial aggregates. pH can be monitored through the fluorescence intensity ratio (FIR) of SNARF-4F. We derived a calibration curve of the FIR as a function of pH. The FIR dynamic responses were measured by adding hydrochloric acid to the buffer solution. Using the developed system, we simultaneously measured the pH changes at two different locations in the SNARF-4F solution. Mitochondrial samples were prepared using optical tweezers to control the number and position of mitochondria. Mitochondrial pH changes (ΔpH) between 0.05 and 0.57 were observed after adding a nutritional supplement (malate and glutamate). In addition, in the comparative experiment on the activities of two mitochondrial populations, the obtained result suggested that the activity differs depending on the difference in the number of mitochondria.
DOI
pH measurements enable the direct monitoring and evaluation of mitochondrial activity. We constructed a scanning near-field optical microscopy system with multioptical fiber probes using the two-photon absorption of a pH-sensitive fluorescent dye, SNARF-4F, to measure the activity difference of mitochondrial aggregates. pH can be monitored through the fluorescence intensity ratio (FIR) of SNARF-4F. We derived a calibration curve of the FIR as a function of pH. The FIR dynamic responses were measured by adding hydrochloric acid to the buffer solution. Using the developed system, we simultaneously measured the pH changes at two different locations in the SNARF-4F solution. Mitochondrial samples were prepared using optical tweezers to control the number and position of mitochondria. Mitochondrial pH changes (ΔpH) between 0.05 and 0.57 were observed after adding a nutritional supplement (malate and glutamate). In addition, in the comparative experiment on the activities of two mitochondrial populations, the obtained result suggested that the activity differs depending on the difference in the number of mitochondria.
DOI
Influence of permittivity on gradient force exerted on Mie spheres
Jun Chen, Kaikai Li, and Xiao Li
In optical trapping, whether a particle could be stably trapped into the focus region greatly depends on the strength of the gradient force. Individual theoretical study on gradient force exerted on a Mie particle is rare because the mathematical separation of the gradient force and the scattering force in the Mie regime is difficult. Based on the recent forces separation work by Du et al. [Sci. Rep. 7, 18042 (2017) [CrossRef] ], we investigate the influence of permittivity (an important macroscopic physical quantity) on the gradient force exerted on a Mie particle by cooperating numerical calculation using fast Fourier transform and analytical analysis using multipole expansion. It is revealed that gradient forces exerted on small spheres are mainly determined by the electric dipole moment except for certain permittivity with which the real part of polarizability of the electric dipole approaches zero, and gradient forces exerted on larger spheres are complex because of the superposition of the multipole moments. The classification of permittivity corresponding to different varying tendencies of gradient forces exerted on small spheres or larger Mie particles are illustrated. Absorption of particles favors the trapping of small spheres by gradient force, while it is bad for the trapping of larger particles. Moreover, the absolute values of the maximal gradient forces exerted on larger Mie particles decline greatly versus the varied imaginary part of permittivity. This work provides elaborate investigation on the different varying tendencies of gradient forces versus permittivity, which favors more accurate and free optical trapping.
DOI
In optical trapping, whether a particle could be stably trapped into the focus region greatly depends on the strength of the gradient force. Individual theoretical study on gradient force exerted on a Mie particle is rare because the mathematical separation of the gradient force and the scattering force in the Mie regime is difficult. Based on the recent forces separation work by Du et al. [Sci. Rep. 7, 18042 (2017) [CrossRef] ], we investigate the influence of permittivity (an important macroscopic physical quantity) on the gradient force exerted on a Mie particle by cooperating numerical calculation using fast Fourier transform and analytical analysis using multipole expansion. It is revealed that gradient forces exerted on small spheres are mainly determined by the electric dipole moment except for certain permittivity with which the real part of polarizability of the electric dipole approaches zero, and gradient forces exerted on larger spheres are complex because of the superposition of the multipole moments. The classification of permittivity corresponding to different varying tendencies of gradient forces exerted on small spheres or larger Mie particles are illustrated. Absorption of particles favors the trapping of small spheres by gradient force, while it is bad for the trapping of larger particles. Moreover, the absolute values of the maximal gradient forces exerted on larger Mie particles decline greatly versus the varied imaginary part of permittivity. This work provides elaborate investigation on the different varying tendencies of gradient forces versus permittivity, which favors more accurate and free optical trapping.
DOI
Extracting the potential-well of a near-field optical trap using the Helmholtz-Hodge decomposition
Mohammad Asif Zaman, Punnag Padhy, Paul C. Hansen, and Lambertus Hesselink
The non-conservative nature of the force field generated by a near-field optical trap is analyzed. A plasmonic C-shaped engraving on a gold film is considered as the trap. The force field is calculated using the Maxwell stress tensor method. The Helmholtz-Hodge decomposition is used to extract the conservative and the non-conservative component of the force. Due to the non-negligible non-conservative component, it is found that the conventional approach of extracting the potential by direct integration of the force is not accurate. Despite the non-conservative nature of the force field, it is found that the statistical properties of a trapped nanoparticle can be estimated from the conservative component of the force field alone. Experimental and numerical results are presented to support the claims.
DOI
The non-conservative nature of the force field generated by a near-field optical trap is analyzed. A plasmonic C-shaped engraving on a gold film is considered as the trap. The force field is calculated using the Maxwell stress tensor method. The Helmholtz-Hodge decomposition is used to extract the conservative and the non-conservative component of the force. Due to the non-negligible non-conservative component, it is found that the conventional approach of extracting the potential by direct integration of the force is not accurate. Despite the non-conservative nature of the force field, it is found that the statistical properties of a trapped nanoparticle can be estimated from the conservative component of the force field alone. Experimental and numerical results are presented to support the claims.
DOI
Friday, April 27, 2018
Photothermal DNA Release from Laser-Tweezed Individual Gold Nanomotors Driven by Photon Angular Momentum
Hana Šípová, Lei Shao, Nils Odebo Länk, Daniel Andrén, and Mikael Käll
Gold nanoparticles offer a unique possibility for contact-free bioanalysis and actuation with high spatial resolution that increases their potential for bioapplications such as affinity-based biosensing, drug delivery, and cancer treatment. Here we demonstrate an ultrasensitive optomechanical method for probing and releasing DNA cargo from individual gold nanoparticles trapped and manipulated by laser tweezers. Single nanorods are operated as rotational nanomotors, driven and controlled by circularly polarized laser light in aqueous solution. By rotational dynamics analysis, we resolve differences in the thickness of adsorbed ultrathin molecular layers, including different DNA conformations, with nanometer resolution. We then utilize photothermal heating to release DNA from single nanomotors while measuring the temperature-dependent kinetics and activation energy of the DNA melting process. The method opens new possibilities for optomechanical quantification and application of thermally induced molecular transitions in strongly confined geometries, such as inside microfluidic devices and single cells.
DOI
Gold nanoparticles offer a unique possibility for contact-free bioanalysis and actuation with high spatial resolution that increases their potential for bioapplications such as affinity-based biosensing, drug delivery, and cancer treatment. Here we demonstrate an ultrasensitive optomechanical method for probing and releasing DNA cargo from individual gold nanoparticles trapped and manipulated by laser tweezers. Single nanorods are operated as rotational nanomotors, driven and controlled by circularly polarized laser light in aqueous solution. By rotational dynamics analysis, we resolve differences in the thickness of adsorbed ultrathin molecular layers, including different DNA conformations, with nanometer resolution. We then utilize photothermal heating to release DNA from single nanomotors while measuring the temperature-dependent kinetics and activation energy of the DNA melting process. The method opens new possibilities for optomechanical quantification and application of thermally induced molecular transitions in strongly confined geometries, such as inside microfluidic devices and single cells.
DOI
Rotation of an optically trapped vaterite microsphere measured using rotational Doppler effect
Xinlin Chen; Guangzong Xiao; Wei Xiong; Kaiyong Yang; Hui Luo; Baoli Yao
The angular velocity of a vaterite microsphere spinning in the optical trap is measured using rotational Doppler effect. The perfectly spherical vaterite microspheres are synthesized via coprecipitation in the presence of silk fibroin nanospheres. When trapped by a circularly polarized beam, the vaterite microsphere is uniformly rotated in the trap center. The probe beams containing two Laguerre–Gaussian beams of opposite topological charge l = ± 7, l = ± 8, and l = ± 9 are illuminated on the spinning vaterite. By analyzing the backscattered light, a frequency shift is observed scaling with the rotation rate of the vaterite microsphere. The multiplicative enhancement of the frequency shift proportion to the topological charge has greatly improved the measurement precision. The reliability and practicability of this approach are verified through varying the topological charge of the probe beam and the trapping laser power. In consideration of the excellent measurement precision of the rotation frequency, this technique might be generally applicable in studying the torsional properties of micro-objects.
DOI
The angular velocity of a vaterite microsphere spinning in the optical trap is measured using rotational Doppler effect. The perfectly spherical vaterite microspheres are synthesized via coprecipitation in the presence of silk fibroin nanospheres. When trapped by a circularly polarized beam, the vaterite microsphere is uniformly rotated in the trap center. The probe beams containing two Laguerre–Gaussian beams of opposite topological charge l = ± 7, l = ± 8, and l = ± 9 are illuminated on the spinning vaterite. By analyzing the backscattered light, a frequency shift is observed scaling with the rotation rate of the vaterite microsphere. The multiplicative enhancement of the frequency shift proportion to the topological charge has greatly improved the measurement precision. The reliability and practicability of this approach are verified through varying the topological charge of the probe beam and the trapping laser power. In consideration of the excellent measurement precision of the rotation frequency, this technique might be generally applicable in studying the torsional properties of micro-objects.
DOI
Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles
Mai Kuboi, Nobuyuki Takeyasu, and Takashi Kaneta
We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.
DOI
We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.
DOI
The viscosity of atmospherically relevant organic particles
Jonathan P. Reid, Allan K. Bertram, David O. Topping, Alexander Laskin, Scot T. Martin, Markus D. Petters, Francis D. Pope & Grazia Rovelli
The importance of organic aerosol particles in the environment has been long established, influencing cloud formation and lifetime, absorbing and scattering sunlight, affecting atmospheric composition and impacting on human health. Conventionally, ambient organic particles were considered to exist as liquids. Recent observations in field measurements and studies in the laboratory suggest that they may instead exist as highly viscous semi-solids or amorphous glassy solids under certain conditions, with important implications for atmospheric chemistry, climate and air quality. This review explores our understanding of aerosol particle phase, particularly as identified by measurements of the viscosity of organic particles, and the atmospheric implications of phase state.
DOI
The importance of organic aerosol particles in the environment has been long established, influencing cloud formation and lifetime, absorbing and scattering sunlight, affecting atmospheric composition and impacting on human health. Conventionally, ambient organic particles were considered to exist as liquids. Recent observations in field measurements and studies in the laboratory suggest that they may instead exist as highly viscous semi-solids or amorphous glassy solids under certain conditions, with important implications for atmospheric chemistry, climate and air quality. This review explores our understanding of aerosol particle phase, particularly as identified by measurements of the viscosity of organic particles, and the atmospheric implications of phase state.
DOI
Migration of blood cells and phospholipid vesicles induced by concentration gradients in microcavities
Saša Vrhovec Hartman, Bojan Božič, Jure Derganc
Microcavities provide a well-controlled flow-free microenvironment and play an important role in many microfluidic systems, for example as cell-culturing microchambers. Here we show that transient concentration gradients that emerge during diffusive exchange of solutes in microcavities induce passive migration (diffusiophoresis) of blood cells and synthetic phospholipid vesicles. The passive migration is observed in various concentration gradients comprising non-electrolytes and electrolytes, i.e., glucose, sucrose, sodium chloride, potassium chloride, potassium benzoate, and potassium sulfate. The results add to prior reports, where gradients of non-electrolytes and monovalent salts, produced by micropipette injection, did not induce a noticeable migration of vesicles. The migration distances measured depended on the solution and the cell or vesicle type, and were in the range of several tens of micrometers. The results show that diffusiophoresis of cells and vesicles is a notable phenomenon in a flow-free environment and has to be taken into account when an accurate spatiotemporal control of cells or vesicles in microcavities is required.
DOI
Microcavities provide a well-controlled flow-free microenvironment and play an important role in many microfluidic systems, for example as cell-culturing microchambers. Here we show that transient concentration gradients that emerge during diffusive exchange of solutes in microcavities induce passive migration (diffusiophoresis) of blood cells and synthetic phospholipid vesicles. The passive migration is observed in various concentration gradients comprising non-electrolytes and electrolytes, i.e., glucose, sucrose, sodium chloride, potassium chloride, potassium benzoate, and potassium sulfate. The results add to prior reports, where gradients of non-electrolytes and monovalent salts, produced by micropipette injection, did not induce a noticeable migration of vesicles. The migration distances measured depended on the solution and the cell or vesicle type, and were in the range of several tens of micrometers. The results show that diffusiophoresis of cells and vesicles is a notable phenomenon in a flow-free environment and has to be taken into account when an accurate spatiotemporal control of cells or vesicles in microcavities is required.
DOI
Laser acceleration of absorbing particles
Tirtha Mitra, Andrew K. Brown, David M. Bernot, Sage Defrances, and Joseph J. Talghader
A Yb-doped fiber laser is used to accelerate and evaporate absorbing particles in air. Optical intensities of 1MW/cm2 and 2MW/cm2 illuminate stainless steel particles. These particles are accelerated to velocities of tens of meters per second before evaporating within a few tenths of a millisecond. Position measurements are made using direct imaging with a high-speed camera. A fundamental system of coupled differential equations to track particle momentum, velocity, mass, radius, temperature, vapor opacity, and temperature distribution is developed and shown to accurately model the trajectories and lifetimes of laser heated particles. Atoms evaporating from the particle impart momentum to the larger particle, which accelerates until it is slowed by drag forces. Heat transfer within the evaporating particles is dominated by radiation diffusion, a process that usually only dominates in astrophysical objects, for example in the photospheres of stars.
DOI
A Yb-doped fiber laser is used to accelerate and evaporate absorbing particles in air. Optical intensities of 1MW/cm2 and 2MW/cm2 illuminate stainless steel particles. These particles are accelerated to velocities of tens of meters per second before evaporating within a few tenths of a millisecond. Position measurements are made using direct imaging with a high-speed camera. A fundamental system of coupled differential equations to track particle momentum, velocity, mass, radius, temperature, vapor opacity, and temperature distribution is developed and shown to accurately model the trajectories and lifetimes of laser heated particles. Atoms evaporating from the particle impart momentum to the larger particle, which accelerates until it is slowed by drag forces. Heat transfer within the evaporating particles is dominated by radiation diffusion, a process that usually only dominates in astrophysical objects, for example in the photospheres of stars.
DOI
Thursday, April 26, 2018
Transverse optical forces and sideways deflections in subwavelength-diameter optical fibers
Tong Xiao, Huakang Yu, Yunyuan Zhang, and Zhiyuan Li
We investigate transverse optical forces exerted on the endface of subwavelength-diameter (SD) optical fiber by using a finite-difference time-domain (FDTD) method. Detailed spatial distributions of transverse optical force along the fiber axis can now be accessible, based on which the dependence of transverse optical force on transverse cross sections, oblique-cut endfaces and high-order mode are carefully studied. Our numerical results demonstrate that either asymmetric cross section or oblique-cut endface would dominantly contribute to the transverse optical force and the corresponding sideways deflection of SD fiber, which is in good agreement with previous experimental observations. The novel behavior of transverse optical force by the high-order mode would give rise to new guidelines for constructing high-performance optomechanical devices.
DOI
We investigate transverse optical forces exerted on the endface of subwavelength-diameter (SD) optical fiber by using a finite-difference time-domain (FDTD) method. Detailed spatial distributions of transverse optical force along the fiber axis can now be accessible, based on which the dependence of transverse optical force on transverse cross sections, oblique-cut endfaces and high-order mode are carefully studied. Our numerical results demonstrate that either asymmetric cross section or oblique-cut endface would dominantly contribute to the transverse optical force and the corresponding sideways deflection of SD fiber, which is in good agreement with previous experimental observations. The novel behavior of transverse optical force by the high-order mode would give rise to new guidelines for constructing high-performance optomechanical devices.
DOI
Determination of the interaction mechanism of 10 µm oil-in-water emulsion droplets using optical tweezers
An Chen, Yu Jing, Fu-Ning Sang, Shao-Wei Li, Jian-Hong Xu
A comprehensive understanding of the stability of an emulsion relies on a quantitative understanding of the dynamic interaction forces between the dispersed emulsion droplets. Microscale droplets have been widely used in various applications, for e.g., in food, cosmetics, controlled drug release, and biology. The dynamic interaction forces between two deformable emulsion droplets in the size range of 20–200 µm have been well-analysed using bubble/drop probe atomic force microscopy, whereas those of the emulsion droplets below 10 µm are rarely studied. In this work, we quantify the interaction forces between a pair of 1,6-hexanediol diacrylate (HDDA) droplets with a 10 µm diameter in an aqueous phase using optical tweezers (NanoTracker™ 2). By capturing two oil droplets in two separate optical traps and bringing them into close proximity, their interaction forces can be measured as a function of the separation distance. The force profiles are analysed using a theoretical model based on the augmented Young–Laplace equation that includes the effects of disjoining pressure. The interaction forces between two 10 µm HDDA oil droplets with SDS molecules adsorbed at their interfaces could be well-described using the classical Derjaguin–Landau−Verwey–Overbeek theory. This work demonstrates a useful methodology to quantify the interaction forces between microscale emulsion droplets with pN force precision, providing a deeper understanding of the stabilization mechanism of O/W emulsions and can be extended to many other emulsion systems.
DOI
A comprehensive understanding of the stability of an emulsion relies on a quantitative understanding of the dynamic interaction forces between the dispersed emulsion droplets. Microscale droplets have been widely used in various applications, for e.g., in food, cosmetics, controlled drug release, and biology. The dynamic interaction forces between two deformable emulsion droplets in the size range of 20–200 µm have been well-analysed using bubble/drop probe atomic force microscopy, whereas those of the emulsion droplets below 10 µm are rarely studied. In this work, we quantify the interaction forces between a pair of 1,6-hexanediol diacrylate (HDDA) droplets with a 10 µm diameter in an aqueous phase using optical tweezers (NanoTracker™ 2). By capturing two oil droplets in two separate optical traps and bringing them into close proximity, their interaction forces can be measured as a function of the separation distance. The force profiles are analysed using a theoretical model based on the augmented Young–Laplace equation that includes the effects of disjoining pressure. The interaction forces between two 10 µm HDDA oil droplets with SDS molecules adsorbed at their interfaces could be well-described using the classical Derjaguin–Landau−Verwey–Overbeek theory. This work demonstrates a useful methodology to quantify the interaction forces between microscale emulsion droplets with pN force precision, providing a deeper understanding of the stabilization mechanism of O/W emulsions and can be extended to many other emulsion systems.
DOI
Optical trapping-Raman spectroscopy (OT-RS) with embedded microscopy imaging for concurrent characterization and monitoring of physical and chemical properties of single particles
Zhiyong Gong, Yong-Le Pan, Gorden Videen, Chuji Wang
The study of physical and chemical properties of a microscopic object, such as a single particle, is made possible using optical trapping (OT) technology combined with other measuring techniques. Here we show a universal optical trap combined with Raman spectroscopy (RS) and microscopy imaging for single-particle studies. The universal optical trap is constructed using two counter-propagating hollow beams and is able to stably levitate single particles of a wide range of properties, such as transparent or absorbing materials, organic (polymers, bioaerosols, etc.) or inorganic constituents (carbon, silica, glass, etc.), and spherical or irregularly shaped morphologies. Both physical and chemical properties and their temporal evolution of the trapped particle can be characterized simultaneously using the integrated OT-RS and imaging system. We created three sample cases to demonstrate the analytical merits of the system: (I) a single particle with no change, (II) partially degraded over the measuring period, and (III) one part from the fragmentized single particle. The particles' chemical compositions, crystalline states, etc. are inferred from their Raman spectra, while their physical properties (sizes, shapes, morphologies, etc.) are revealed by images. This integrated OT-RS system provides a new approach to concurrently characterize and monitor physical and chemical properties of single micrometer-sized objects optically trapped in air.
DOI
The study of physical and chemical properties of a microscopic object, such as a single particle, is made possible using optical trapping (OT) technology combined with other measuring techniques. Here we show a universal optical trap combined with Raman spectroscopy (RS) and microscopy imaging for single-particle studies. The universal optical trap is constructed using two counter-propagating hollow beams and is able to stably levitate single particles of a wide range of properties, such as transparent or absorbing materials, organic (polymers, bioaerosols, etc.) or inorganic constituents (carbon, silica, glass, etc.), and spherical or irregularly shaped morphologies. Both physical and chemical properties and their temporal evolution of the trapped particle can be characterized simultaneously using the integrated OT-RS and imaging system. We created three sample cases to demonstrate the analytical merits of the system: (I) a single particle with no change, (II) partially degraded over the measuring period, and (III) one part from the fragmentized single particle. The particles' chemical compositions, crystalline states, etc. are inferred from their Raman spectra, while their physical properties (sizes, shapes, morphologies, etc.) are revealed by images. This integrated OT-RS system provides a new approach to concurrently characterize and monitor physical and chemical properties of single micrometer-sized objects optically trapped in air.
DOI
Force distribution inside the spoof surface plasmon polariton waveguide
M. Aghadjani, M. Erementchouk, and P. Mazumder
The Maxwell stress tensor for the field inside a spoof surface plasmon polariton (SSPP) waveguide and the electromagnetic force distribution acting on a dielectric particle are investigated. We show that, for particles made of material with the dielectric function slightly different from that of the medium filling the waveguide, the force distribution is fully described by the SSPP field in the absence of the particle. The spatial profile of the field strongly depends on the relation between the operating frequency and the SSPP resonance frequency. We show that varying the characteristic frequencies introduces a switching effect, when the direction of the force along the waveguide axis changes (from toward the grooves to away from them). We propose a pumping mechanism based on force acting on a small particle inside the SSPP waveguide.
DOI
The Maxwell stress tensor for the field inside a spoof surface plasmon polariton (SSPP) waveguide and the electromagnetic force distribution acting on a dielectric particle are investigated. We show that, for particles made of material with the dielectric function slightly different from that of the medium filling the waveguide, the force distribution is fully described by the SSPP field in the absence of the particle. The spatial profile of the field strongly depends on the relation between the operating frequency and the SSPP resonance frequency. We show that varying the characteristic frequencies introduces a switching effect, when the direction of the force along the waveguide axis changes (from toward the grooves to away from them). We propose a pumping mechanism based on force acting on a small particle inside the SSPP waveguide.
DOI
Optical trapping and micromanipulation with a photonic lantern-mode multiplexer
Amado M. Velázquez-Benítez, K. Yanín Guerra-Santillán, Raúl Caudillo-Viurquez, J. Enrique Antonio-López, Rodrigo Amezcua-Correa, and Juan Hernández-Cordero
We demonstrate a simple approach based on a photonic lantern spatial-mode multiplexer and a few-mode fiber for optical and manipulation of multiple microspheres. Selective generation of linearly polarized (LP) fiber modes provides light patterns useful for trapping one or multiple microparticles. Furthermore, rotation of the particles can be achieved by switching between degenerate LP modes, as well as through polarization rotation of the input light. Our results show that emerging fiber optic devices such as photonic lanterns can provide a versatile and compact means for developing optical fiber traps.
DOI
We demonstrate a simple approach based on a photonic lantern spatial-mode multiplexer and a few-mode fiber for optical and manipulation of multiple microspheres. Selective generation of linearly polarized (LP) fiber modes provides light patterns useful for trapping one or multiple microparticles. Furthermore, rotation of the particles can be achieved by switching between degenerate LP modes, as well as through polarization rotation of the input light. Our results show that emerging fiber optic devices such as photonic lanterns can provide a versatile and compact means for developing optical fiber traps.
DOI
Red blood cell membrane damage by light induced thermal gradient under optical trap
Aniket Chowdhury Deepak Waghmare Raktim Dasgupta Shovan Kumar Majumder
Rapid membrane damage of optically trapped red blood cells was observed at trapping powers ≥ 280 mW. An excellent agreement between the estimated laser induced thermal gradient across trapped cell's membrane and that typically required for membrane electropermeabilization suggests a mechanism involving temperature gradient induced electropermeabilization of membrane. Also the rapid collapse of the trapped cell due to membrane rupture was seen to cause shock waves in the surroundings permeabilizing nearby untrapped cells. When the experiments were carried out with red blood cells collected from type II diabetic patients a noticeable change in the damage rate compared to normal red blood cells was seen suggesting a novel optical diagnosis method for the disease. Near infrared trap laser induced membrane damage and subsequent rapture of human red blood cells.
DOI
Rapid membrane damage of optically trapped red blood cells was observed at trapping powers ≥ 280 mW. An excellent agreement between the estimated laser induced thermal gradient across trapped cell's membrane and that typically required for membrane electropermeabilization suggests a mechanism involving temperature gradient induced electropermeabilization of membrane. Also the rapid collapse of the trapped cell due to membrane rupture was seen to cause shock waves in the surroundings permeabilizing nearby untrapped cells. When the experiments were carried out with red blood cells collected from type II diabetic patients a noticeable change in the damage rate compared to normal red blood cells was seen suggesting a novel optical diagnosis method for the disease. Near infrared trap laser induced membrane damage and subsequent rapture of human red blood cells.
DOI
Studying effect of carrier fluid viscosity in magnetite based ferrofluids using optical tweezers
S Savitha, Shruthi S Iyengar, Sharath Ananthamurthy and Sarbari Bhattacharya
Ferrofluids with varying viscosities of carrier fluids have been prepared with magnetite (Fe3O4) nanoparticles. The nanoparticles were synthesized by chemical co-precipitation and characterized using X-Ray Diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM). They were found to be nearly spherical in shape with an almost uniform size of 13nm. The superparamagnetic nature of the water based ferrofluids at room temperature was established by SQUID magnetometry. Dynamic light scattering (DLS) was carried out to establish the size of the nanoparticle clusters in the ferrofluids synthesized. The results indicate an increase in cluster size with increase in carrier fluid viscosity. This is supported by results from Raman Spectroscopy. A further attempt to characterise these ferrofluids was made by studying the behaviour of well characterised non-magnetic micron sized probes that are optically trapped while suspended in the ferrofluid. An increase in carrier fluid viscosity results in a decrease in corner frequency when only the carrier fluid is used as the suspending medium. When the magnetic component is also present the corner frequency is higher than with just the carrier fluid. This relative increase happens at all laser powers at the trapping plane. This trend is also found to be independent of the size and material of the probe particle. Comparisons of various parameters that influence optical trapping lead us to believe that the enhancement could be due to a directed motion of the magnetic clusters in the presence of an optical trap.
DOI
Ferrofluids with varying viscosities of carrier fluids have been prepared with magnetite (Fe3O4) nanoparticles. The nanoparticles were synthesized by chemical co-precipitation and characterized using X-Ray Diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM). They were found to be nearly spherical in shape with an almost uniform size of 13nm. The superparamagnetic nature of the water based ferrofluids at room temperature was established by SQUID magnetometry. Dynamic light scattering (DLS) was carried out to establish the size of the nanoparticle clusters in the ferrofluids synthesized. The results indicate an increase in cluster size with increase in carrier fluid viscosity. This is supported by results from Raman Spectroscopy. A further attempt to characterise these ferrofluids was made by studying the behaviour of well characterised non-magnetic micron sized probes that are optically trapped while suspended in the ferrofluid. An increase in carrier fluid viscosity results in a decrease in corner frequency when only the carrier fluid is used as the suspending medium. When the magnetic component is also present the corner frequency is higher than with just the carrier fluid. This relative increase happens at all laser powers at the trapping plane. This trend is also found to be independent of the size and material of the probe particle. Comparisons of various parameters that influence optical trapping lead us to believe that the enhancement could be due to a directed motion of the magnetic clusters in the presence of an optical trap.
DOI
Tuesday, April 24, 2018
Optical trapping of single quantum dots for cavity quantum electrodynamics
Pengfei Zhang, Gang Song, and Li Yu
We report here a nanostructure that traps single quantum dots for studying strong cavity-emitter coupling. The nanostructure is designed with two elliptical holes in a thin silver patch and a slot that connects the holes. This structure has two functionalities: (1) tweezers for optical trapping; (2) a plasmonic resonant cavity for quantum electrodynamics. The electromagnetic response of the cavity is calculated by finite-difference time-domain (FDTD) simulations, and the optical force is characterized based on the Maxwell’s stress tensor method. To be tweezers, this structure tends to trap quantum dots at the edges of its tips where light is significantly confined. To be a plasmonic cavity, its plasmonic resonant mode interacts strongly with the trapped quantum dots due to the enhanced electric field. Rabi splitting and anti-crossing phenomena are observed in the calculated scattering spectra, demonstrating that a strong-coupling regime has been achieved. The method present here provides a robust way to position a single quantum dot in a nanocavity for investigating cavity quantum electrodynamics.
DOI
We report here a nanostructure that traps single quantum dots for studying strong cavity-emitter coupling. The nanostructure is designed with two elliptical holes in a thin silver patch and a slot that connects the holes. This structure has two functionalities: (1) tweezers for optical trapping; (2) a plasmonic resonant cavity for quantum electrodynamics. The electromagnetic response of the cavity is calculated by finite-difference time-domain (FDTD) simulations, and the optical force is characterized based on the Maxwell’s stress tensor method. To be tweezers, this structure tends to trap quantum dots at the edges of its tips where light is significantly confined. To be a plasmonic cavity, its plasmonic resonant mode interacts strongly with the trapped quantum dots due to the enhanced electric field. Rabi splitting and anti-crossing phenomena are observed in the calculated scattering spectra, demonstrating that a strong-coupling regime has been achieved. The method present here provides a robust way to position a single quantum dot in a nanocavity for investigating cavity quantum electrodynamics.
DOI
Randomizing phase to remove acousto-optic device wiggle errors for high-resolution optical tweezers
Andrew G. Baker, Cho-Ying Chuang, Miles Whitmore, and Matthew J. Comstock
Acousto-optic (AO) devices have been used extensively in optical tweezers because of their flexibility and speed; however, these devices have trap positioning inaccuracies that limit their usefulness, especially for high-resolution applications. We show that these inaccuracies are due to interference patterns within the AO device sound fields. We have devised a method that removes these inaccuracies by reducing the coherence of the sound fields by directly controlling and randomizing the phase of the radio frequency voltage input signal. We demonstrate that the trapping inaccuracies are eliminated, for both constant trap position and force-ramp measurements, and that no additional noise is added. We show that this random phase method is applicable to both acousto-optic modulator and deflector type devices and can be easily integrated via software upgrade into existing instruments.
DOI
Acousto-optic (AO) devices have been used extensively in optical tweezers because of their flexibility and speed; however, these devices have trap positioning inaccuracies that limit their usefulness, especially for high-resolution applications. We show that these inaccuracies are due to interference patterns within the AO device sound fields. We have devised a method that removes these inaccuracies by reducing the coherence of the sound fields by directly controlling and randomizing the phase of the radio frequency voltage input signal. We demonstrate that the trapping inaccuracies are eliminated, for both constant trap position and force-ramp measurements, and that no additional noise is added. We show that this random phase method is applicable to both acousto-optic modulator and deflector type devices and can be easily integrated via software upgrade into existing instruments.
DOI
Single Particle Differentiation through 2D Optical Fiber Trapping and Back-Scattered Signal Statistical Analysis: An Exploratory Approach
Joana S. Paiva, Rita S. R. Ribeiro, João P. S. Cunha, Carla C. Rosa and Pedro A. S. Jorge
Recent trends on microbiology point out the urge to develop optical micro-tools with multifunctionalities such as simultaneous manipulation and sensing. Considering that miniaturization has been recognized as one of the most important paradigms of emerging sensing biotechnologies, optical fiber tools, including Optical Fiber Tweezers (OFTs), are suitable candidates for developing multifunctional small sensors for Medicine and Biology. OFTs are flexible and versatile optotools based on fibers with one extremity patterned to form a micro-lens. These are able to focus laser beams and exert forces onto microparticles strong enough (piconewtons) to trap and manipulate them. In this paper, through an exploratory analysis of a 45 features set, including time and frequency-domain parameters of the back-scattered signal of particles trapped by a polymeric lens, we created a novel single feature able to differentiate synthetic particles (PMMA and Polystyrene) from living yeasts cells. This single statistical feature can be useful for the development of label-free hybrid optical fiber sensors with applications in infectious diseases detection or cells sorting. It can also contribute, by revealing the most significant information that can be extracted from the scattered signal, to the development of a simpler method for particles characterization (in terms of composition, heterogeneity degree) than existent technologies.
DOI
Recent trends on microbiology point out the urge to develop optical micro-tools with multifunctionalities such as simultaneous manipulation and sensing. Considering that miniaturization has been recognized as one of the most important paradigms of emerging sensing biotechnologies, optical fiber tools, including Optical Fiber Tweezers (OFTs), are suitable candidates for developing multifunctional small sensors for Medicine and Biology. OFTs are flexible and versatile optotools based on fibers with one extremity patterned to form a micro-lens. These are able to focus laser beams and exert forces onto microparticles strong enough (piconewtons) to trap and manipulate them. In this paper, through an exploratory analysis of a 45 features set, including time and frequency-domain parameters of the back-scattered signal of particles trapped by a polymeric lens, we created a novel single feature able to differentiate synthetic particles (PMMA and Polystyrene) from living yeasts cells. This single statistical feature can be useful for the development of label-free hybrid optical fiber sensors with applications in infectious diseases detection or cells sorting. It can also contribute, by revealing the most significant information that can be extracted from the scattered signal, to the development of a simpler method for particles characterization (in terms of composition, heterogeneity degree) than existent technologies.
DOI
The tug-of-war behavior of a Brownian particle in an asymmetric double optical trap with stochastic fluctuations
FeiLong Jia-PeiZhu
A Brownian particle optically trapped in an asymmetric double potential surrounded by a thermal bath was simulated. Under the cooperative action of the resultant deterministic optical force and the stochastic fluctuations of the thermal bath, the confined particle undergoes Kramers transition, and oscillates between the two traps with a probability of trap occupancy that is asymmetrically distributed about the midpoint. The simulation results obtained at different temperatures indicate that the oscillation behavior of the particle can be treated as the result of a tug-of-war game played between the resultant deterministic force and the random force. We also employ a bistable model to explain the observed phenomena.
DOI
A Brownian particle optically trapped in an asymmetric double potential surrounded by a thermal bath was simulated. Under the cooperative action of the resultant deterministic optical force and the stochastic fluctuations of the thermal bath, the confined particle undergoes Kramers transition, and oscillates between the two traps with a probability of trap occupancy that is asymmetrically distributed about the midpoint. The simulation results obtained at different temperatures indicate that the oscillation behavior of the particle can be treated as the result of a tug-of-war game played between the resultant deterministic force and the random force. We also employ a bistable model to explain the observed phenomena.
DOI
Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement
Y. Z. Shi, S. Xiong, Y. Zhang, L. K. Chin, Y. –Y. Chen, J. B. Zhang, T. H. Zhang, W. Ser, A. Larson, L. S. Hoi, J. H. Wu, T. N. Chen, Z. C. Yang, Y. L. Hao, B. Liedberg, P. H. Yap, D. P. Tsai, C.-W. Qiu & A. Q. Liu
Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer’s theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level.
DOI
Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer’s theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level.
DOI
Accounting for polarization in the calibration of a donut beam axial optical tweezers
Russell Pollari, Joshua N. Milstein
Advances in light shaping techniques are leading to new tools for optical trapping and micromanipulation. For example, optical tweezers made from Laguerre-Gaussian or donut beams display an increased axial trap strength and can impart angular momentum to rotate a specimen. However, the application of donut beam optical tweezers to precision, biophysical measurements remains limited due to a lack of methods for calibrating such devices sufficiently. For instance, one notable complication, not present when trapping with a Gaussian beam, is that the polarization of the trap light can significantly affect the tweezers’ strength as well as the location of the trap. In this article, we show how to precisely calibrate the axial trap strength as a function of height above the coverslip surface while accounting for focal shifts in the trap position arising from radiation pressure, mismatches in the index of refraction, and polarization induced intensity variations. This provides a foundation for implementing a donut beam optical tweezers capable of applying precise axial forces.
DOI
Advances in light shaping techniques are leading to new tools for optical trapping and micromanipulation. For example, optical tweezers made from Laguerre-Gaussian or donut beams display an increased axial trap strength and can impart angular momentum to rotate a specimen. However, the application of donut beam optical tweezers to precision, biophysical measurements remains limited due to a lack of methods for calibrating such devices sufficiently. For instance, one notable complication, not present when trapping with a Gaussian beam, is that the polarization of the trap light can significantly affect the tweezers’ strength as well as the location of the trap. In this article, we show how to precisely calibrate the axial trap strength as a function of height above the coverslip surface while accounting for focal shifts in the trap position arising from radiation pressure, mismatches in the index of refraction, and polarization induced intensity variations. This provides a foundation for implementing a donut beam optical tweezers capable of applying precise axial forces.
DOI
Acousto-optical tweezers for stretch of DNA molecule
Thanh Thai Doan, Khoa Doan Quoc, Quy Ho Quang
The appearance of microlenses in the amorphous crystal Ge33As12Se33 modulated by the acoustic waves with intensity of 107 W/m2 and frequency of (200–400) MHz is presented. The flexibility of microlens, i.e. the dependence of its focal point in 3D space on the parameters of acoustic wave is studied. Basing on flexibility of microlens, a model of acousto-optical tweezers to stretch the λ-phage WLC DNA molecule is proposed. And then the control process of stretched length of DNA molecule in 3D space of the water (fluid) by calibration of the acoustic frequency is numerically observed and discussed. The obtained results show the possibility to use the acousto-optical tweezers to control the stretched length of WLC DNA molecule with high fineness.
DOI
The appearance of microlenses in the amorphous crystal Ge33As12Se33 modulated by the acoustic waves with intensity of 107 W/m2 and frequency of (200–400) MHz is presented. The flexibility of microlens, i.e. the dependence of its focal point in 3D space on the parameters of acoustic wave is studied. Basing on flexibility of microlens, a model of acousto-optical tweezers to stretch the λ-phage WLC DNA molecule is proposed. And then the control process of stretched length of DNA molecule in 3D space of the water (fluid) by calibration of the acoustic frequency is numerically observed and discussed. The obtained results show the possibility to use the acousto-optical tweezers to control the stretched length of WLC DNA molecule with high fineness.
DOI
Friday, April 20, 2018
Reconfigurable Sorting of Nanoparticles on a Thermal Tuning Silicon Based Optofluidic Chip
Xiaofu Xu, Yuming Dong, Guanghui Wang , Wenxiang Jiao, Zhoufeng Ying, Ho-pui Ho, Xuping Zhang
We numerically demonstrate a scheme of optofluidic sorting of nanoparticles on a silicon-based lab-on-a-chip system, with reconfigurable and multilevel sorting size thresholds. Size-dependent sorting of nanoparticles originates from the size-dependent distribution of trapping potential wells, which determines whether a nanoparticle can jump back and forth between parallel waveguides in weakly coupling condition. In numerical modeling, we find that it is easier for larger ones to transfer and the size threshold is power ratio dependent. By setting a thermal tuning ring-assisted Mach-Zehnder interferometer ahead, we can adjust effectively the input power ratio of the parallel waveguides as well as the potential well distribution across them, working as a thermal tuning reconfigurable sorting unit. Consequently, a tree cascaded unit with different thresholds is also presented to be a reconfigurable multilevel sorting unit. The proposed design offers a simple and ultracompact scheme for the reconfigurable and multilevel optofluidic sorting of nanoparticles on thermo-optofluidics chip for the first time.
DOI
We numerically demonstrate a scheme of optofluidic sorting of nanoparticles on a silicon-based lab-on-a-chip system, with reconfigurable and multilevel sorting size thresholds. Size-dependent sorting of nanoparticles originates from the size-dependent distribution of trapping potential wells, which determines whether a nanoparticle can jump back and forth between parallel waveguides in weakly coupling condition. In numerical modeling, we find that it is easier for larger ones to transfer and the size threshold is power ratio dependent. By setting a thermal tuning ring-assisted Mach-Zehnder interferometer ahead, we can adjust effectively the input power ratio of the parallel waveguides as well as the potential well distribution across them, working as a thermal tuning reconfigurable sorting unit. Consequently, a tree cascaded unit with different thresholds is also presented to be a reconfigurable multilevel sorting unit. The proposed design offers a simple and ultracompact scheme for the reconfigurable and multilevel optofluidic sorting of nanoparticles on thermo-optofluidics chip for the first time.
DOI
Development of a collision-avoidance vector based control algorithm for automated in-vivo transportation of biological cells
Xiaojian Li, Shuxun Chen, Chichi Liu, Shuk Han Cheng, Yong Wang, Dong Sun
With the rapid development of precision medicine, the in-vivo manipulation of microparticles has attracted increased attention in recent years. Collision is a main cause of the failure of in-vivo particle transportation. In this paper, an automated control approach with obstacle avoidance function is proposed for in-vivo cell transportation. In the proposed approach, a collision-avoidance vector method is utilized to avoid obstacles during the transportation of the target cell. The proposed method integrates obstacle detection and collision avoidance into a single step, hence reducing the duration of online processing while enhancing the accuracy of obstacle detection. With the proposed approach, different collision avoidance strategies are designed to suit for different transportation environments. The proposed approach exhibits the advantages of reduced online calculation, fast response, high accuracy, and disturbance compensation. Experiments are performed to demonstrate the effectiveness of the proposed controller.
DOI
With the rapid development of precision medicine, the in-vivo manipulation of microparticles has attracted increased attention in recent years. Collision is a main cause of the failure of in-vivo particle transportation. In this paper, an automated control approach with obstacle avoidance function is proposed for in-vivo cell transportation. In the proposed approach, a collision-avoidance vector method is utilized to avoid obstacles during the transportation of the target cell. The proposed method integrates obstacle detection and collision avoidance into a single step, hence reducing the duration of online processing while enhancing the accuracy of obstacle detection. With the proposed approach, different collision avoidance strategies are designed to suit for different transportation environments. The proposed approach exhibits the advantages of reduced online calculation, fast response, high accuracy, and disturbance compensation. Experiments are performed to demonstrate the effectiveness of the proposed controller.
DOI
Flying particle microlaser and temperature sensor in hollow-core photonic crystal fiber
Richard Zeltner, Riccardo Pennetta, Shangran Xie, and Philip St.J. Russell
Whispering-gallery mode (WGM) resonators combine small optical mode volumes with narrow resonance linewidths, making them exciting platforms for a variety of applications. Here we report a flying WGM microlaser, realized by optically trapping a dye-doped microparticle within a liquid-filled hollow-core photonic crystal fiber (HC-PCF) using a CW laser and then pumping it with a pulsed excitation laser whose wavelength matches the absorption band of the dye. The laser emits into core-guided modes that can be detected at the endfaces of the HC-PCF. Using radiation forces, the microlaser can be freely propelled along the HC-PCF over multi-centimeter distances—orders of magnitude farther than in previous experiments where tweezers and fiber traps were used. The system can be used to measure temperature with high spatial resolution, by exploiting the temperature-dependent frequency shift of the lasing modes, and may also permit precise delivery of light to remote locations.
DOI
Whispering-gallery mode (WGM) resonators combine small optical mode volumes with narrow resonance linewidths, making them exciting platforms for a variety of applications. Here we report a flying WGM microlaser, realized by optically trapping a dye-doped microparticle within a liquid-filled hollow-core photonic crystal fiber (HC-PCF) using a CW laser and then pumping it with a pulsed excitation laser whose wavelength matches the absorption band of the dye. The laser emits into core-guided modes that can be detected at the endfaces of the HC-PCF. Using radiation forces, the microlaser can be freely propelled along the HC-PCF over multi-centimeter distances—orders of magnitude farther than in previous experiments where tweezers and fiber traps were used. The system can be used to measure temperature with high spatial resolution, by exploiting the temperature-dependent frequency shift of the lasing modes, and may also permit precise delivery of light to remote locations.
DOI
Optical fiber tips for biological applications: From light confinement, biosensing to bioparticles manipulation
Joana S. Paiva, Pedro A. S. Jorge, Carla C. Rosa, João P. S. Cunha
The tip of an optical fiber has been considered an attractive platform in Biology. The simple cleaved end of an optical fiber can be machined, patterned and/or functionalized, acquiring unique properties enabling the exploitation of novel optical phenomena. Prompted by the constant need to measure and manipulate nanoparticles, the invention of the Scanning Near-field Optical Microscopy (SNOM) triggered the optimization and development of novel fiber tip microfabrication methods. In fact, the fiber tip was soon considered a key element in SNOM by confining light to sufficiently small extensions, challenging the diffraction limit. As result and in consequence of the newly proposed “Lab On Tip” concept, several geometries of fiber tips were applied in three main fields: imaging (in Microscopy/Spectroscopy), biosensors and micromanipulation (Optical Fiber Tweezers, OFTs). These are able to exert forces on microparticles, trap and manipulate them for relevant applications, as biomolecules mechanical study or protein aggregates unfolding. This review presents an overview of the main achievements, most impactful studies and limitations of fiber tip-based configurations within the above three fields, along the past 10 years. OFTs could be in future a valuable tool for studying several cellular phenomena such as neurodegeneration caused by abnormal protein fibrils or manipulating organelles within cells. This could contribute to understand the mechanisms of some diseases or biophenomena, as the axonal growth in neurons. To the best of our knowledge, no other review article has so far provided such a broad view. Despite of the limitations, fiber tips have key roles in Biology/Medicine.
DOI
The tip of an optical fiber has been considered an attractive platform in Biology. The simple cleaved end of an optical fiber can be machined, patterned and/or functionalized, acquiring unique properties enabling the exploitation of novel optical phenomena. Prompted by the constant need to measure and manipulate nanoparticles, the invention of the Scanning Near-field Optical Microscopy (SNOM) triggered the optimization and development of novel fiber tip microfabrication methods. In fact, the fiber tip was soon considered a key element in SNOM by confining light to sufficiently small extensions, challenging the diffraction limit. As result and in consequence of the newly proposed “Lab On Tip” concept, several geometries of fiber tips were applied in three main fields: imaging (in Microscopy/Spectroscopy), biosensors and micromanipulation (Optical Fiber Tweezers, OFTs). These are able to exert forces on microparticles, trap and manipulate them for relevant applications, as biomolecules mechanical study or protein aggregates unfolding. This review presents an overview of the main achievements, most impactful studies and limitations of fiber tip-based configurations within the above three fields, along the past 10 years. OFTs could be in future a valuable tool for studying several cellular phenomena such as neurodegeneration caused by abnormal protein fibrils or manipulating organelles within cells. This could contribute to understand the mechanisms of some diseases or biophenomena, as the axonal growth in neurons. To the best of our knowledge, no other review article has so far provided such a broad view. Despite of the limitations, fiber tips have key roles in Biology/Medicine.
DOI
Plasmonic Spherical Heterodimers: Reversal of Optical Binding Force Based on the Forced Breaking of Symmetry
M. R. C. Mahdy, Md. Danesh, Tianhang Zhang, Weiqiang Ding, Hamim Mahmud Rivy, Ariful Bari Chowdhury & M. Q. Mehmood
The stimulating connection between the reversal of near-field plasmonic binding force and the role of symmetry-breaking has not been investigated comprehensively in the literature. In this work, the symmetry of spherical plasmonic heterodimer-setup is broken forcefully by shining the light from a specific side of the set-up instead of impinging it from the top. We demonstrate that for the forced symmetry-broken spherical heterodimer-configurations: reversal of lateral and longitudinal near-field binding force follow completely distinct mechanisms. Interestingly, the reversal of longitudinal binding force can be easily controlled either by changing the direction of light propagation or by varying their relative orientation. This simple process of controlling binding force may open a novel generic way of optical manipulation even with the heterodimers of other shapes. Though it is commonly believed that the reversal of near-field plasmonic binding force should naturally occur for the presence of bonding and anti-bonding modes or at least for the Fano resonance (and plasmonic forces mostly arise from the surface force), our study based on Lorentz-force dynamics suggests notably opposite proposals for the aforementioned cases. Observations in this article can be very useful for improved sensors, particle clustering and aggregation.
DOI
The stimulating connection between the reversal of near-field plasmonic binding force and the role of symmetry-breaking has not been investigated comprehensively in the literature. In this work, the symmetry of spherical plasmonic heterodimer-setup is broken forcefully by shining the light from a specific side of the set-up instead of impinging it from the top. We demonstrate that for the forced symmetry-broken spherical heterodimer-configurations: reversal of lateral and longitudinal near-field binding force follow completely distinct mechanisms. Interestingly, the reversal of longitudinal binding force can be easily controlled either by changing the direction of light propagation or by varying their relative orientation. This simple process of controlling binding force may open a novel generic way of optical manipulation even with the heterodimers of other shapes. Though it is commonly believed that the reversal of near-field plasmonic binding force should naturally occur for the presence of bonding and anti-bonding modes or at least for the Fano resonance (and plasmonic forces mostly arise from the surface force), our study based on Lorentz-force dynamics suggests notably opposite proposals for the aforementioned cases. Observations in this article can be very useful for improved sensors, particle clustering and aggregation.
DOI
Revolving supramolecular chiral structures powered by light in nanomotor-doped liquid crystals
Tetiana Orlova, Federico Lancia, Charles Loussert, Supitchaya Iamsaard, Nathalie Katsonis & Etienne Brasselet
Molecular machines operated by light have been recently shown to be able to produce oriented motion at the molecular scale1,2 as well as do macroscopic work when embedded in supramolecular structures3,4,5. However, any supramolecular movement irremediably ceases as soon as the concentration of the interconverting molecular motors or switches reaches a photo-stationary state6,7. To circumvent this limitation, researchers have typically relied on establishing oscillating illumination conditions—either by modulating the source intensity8,9 or by using bespoke illumination arrangements10,11,12,13. In contrast, here we report a supramolecular system in which the emergence of oscillating patterns is encoded at the molecular level. Our system comprises chiral liquid crystal structures that revolve continuously when illuminated, under the action of embedded light-driven molecular motors. The rotation at the supramolecular level is sustained by the diffusion of the motors away from a localized illumination area. Above a critical irradiation power, we observe a spontaneous symmetry breaking that dictates the directionality of the supramolecular rotation. The interplay between the twist of the supramolecular structure and the diffusion14 of the chiral molecular motors creates continuous, regular and unidirectional rotation of the liquid crystal structure under non-equilibrium conditions.
DOI
Molecular machines operated by light have been recently shown to be able to produce oriented motion at the molecular scale1,2 as well as do macroscopic work when embedded in supramolecular structures3,4,5. However, any supramolecular movement irremediably ceases as soon as the concentration of the interconverting molecular motors or switches reaches a photo-stationary state6,7. To circumvent this limitation, researchers have typically relied on establishing oscillating illumination conditions—either by modulating the source intensity8,9 or by using bespoke illumination arrangements10,11,12,13. In contrast, here we report a supramolecular system in which the emergence of oscillating patterns is encoded at the molecular level. Our system comprises chiral liquid crystal structures that revolve continuously when illuminated, under the action of embedded light-driven molecular motors. The rotation at the supramolecular level is sustained by the diffusion of the motors away from a localized illumination area. Above a critical irradiation power, we observe a spontaneous symmetry breaking that dictates the directionality of the supramolecular rotation. The interplay between the twist of the supramolecular structure and the diffusion14 of the chiral molecular motors creates continuous, regular and unidirectional rotation of the liquid crystal structure under non-equilibrium conditions.
DOI
Giant and tunable optical torque for micro-motors by increased force arm and resonantly enhanced force
Yong Geng, Jiubin Tan, Yongyin Cao, Yixuan Zhao, Zhengjun Liu & Weiqiang Ding
Micro-motors driven by light field have attracted much attentions for their potential applications. In order to drive the rotation of a micro-motor, structured optical beams with orbital angular momentum, spin angular momentum, anisotropic medium, and/or inhomogeneous intensity distribution should be used. Even though, it is still challenge to increase the optical torques (OT) in a flexible and controllable way in case of moderate incident power. In this paper, a new scheme achieving giant optical torque is proposed by increasing both the force arm and the force amplitude with the assistance of a ring resonator. In this case, the optical torque doesn’t act on the target directly by the incident beam, but is transmitted to it by rotating the ring resonator connected with it. Using the finite-difference in time-domain method, we calculate the optical torque and find that both the direction and the amplitude of the torque can be tuned flexibly by modifying the frequency, or the relative phases of the sources. More importantly, the optical torque obtained here by linearly polarized beams can be 3 orders larger than those obtained using the structured beams. This opt-mechanical-resonator based optical torque engineering system may find potential applications in optical driven micro-machines.
DOI
Micro-motors driven by light field have attracted much attentions for their potential applications. In order to drive the rotation of a micro-motor, structured optical beams with orbital angular momentum, spin angular momentum, anisotropic medium, and/or inhomogeneous intensity distribution should be used. Even though, it is still challenge to increase the optical torques (OT) in a flexible and controllable way in case of moderate incident power. In this paper, a new scheme achieving giant optical torque is proposed by increasing both the force arm and the force amplitude with the assistance of a ring resonator. In this case, the optical torque doesn’t act on the target directly by the incident beam, but is transmitted to it by rotating the ring resonator connected with it. Using the finite-difference in time-domain method, we calculate the optical torque and find that both the direction and the amplitude of the torque can be tuned flexibly by modifying the frequency, or the relative phases of the sources. More importantly, the optical torque obtained here by linearly polarized beams can be 3 orders larger than those obtained using the structured beams. This opt-mechanical-resonator based optical torque engineering system may find potential applications in optical driven micro-machines.
DOI
Wednesday, April 18, 2018
Electro-optic deflectors deliver advantages over acousto-optical deflectors in a high resolution, ultra-fast force-clamp optical trap
Michael S. Woody, Marco Capitanio, E. Michael Ostap, and Yale E. Goldman
We characterized experimental artifacts arising from the non-linear response of acousto-optical deflectors (AODs) in an ultra-fast force-clamp optical trap and have shown that using electro-optical deflectors (EODs) instead eliminates these artifacts. We give an example of the effects of these artifacts in our ultra-fast force clamp studies of the interaction of myosin with actin filaments. The experimental setup, based on the concept of Capitanio et al. [Nat. Methods 9, 1013–1019 (2012)] utilizes a bead-actin-bead dumbbell held in two force-clamped optical traps which apply a load to the dumbbell to move it at a constant velocity. When myosin binds to actin, the filament motion stops quickly as the total force from the optical traps is transferred to the actomyosin attachment. We found that in our setup, AODs were unsuitable for beam steering due to non-linear variations in beam intensity and deflection angle as a function of driving frequency, likely caused by low-amplitude standing acoustic waves in the deflectors. These aberrations caused instability in the force feedback loops leading to artifactual jumps in the trap position. We demonstrate that beam steering with EODs improves the performance of our instrument. Combining the superior beam-steering capability of the EODs, force acquisition via back-focal-plane interferometry, and dual high-speed FPGA-based feedback loops, we apply precise and constant loads to study the dynamics of interactions between actin and myosin. The same concept applies to studies of other biomolecular interactions.
DOI
We characterized experimental artifacts arising from the non-linear response of acousto-optical deflectors (AODs) in an ultra-fast force-clamp optical trap and have shown that using electro-optical deflectors (EODs) instead eliminates these artifacts. We give an example of the effects of these artifacts in our ultra-fast force clamp studies of the interaction of myosin with actin filaments. The experimental setup, based on the concept of Capitanio et al. [Nat. Methods 9, 1013–1019 (2012)] utilizes a bead-actin-bead dumbbell held in two force-clamped optical traps which apply a load to the dumbbell to move it at a constant velocity. When myosin binds to actin, the filament motion stops quickly as the total force from the optical traps is transferred to the actomyosin attachment. We found that in our setup, AODs were unsuitable for beam steering due to non-linear variations in beam intensity and deflection angle as a function of driving frequency, likely caused by low-amplitude standing acoustic waves in the deflectors. These aberrations caused instability in the force feedback loops leading to artifactual jumps in the trap position. We demonstrate that beam steering with EODs improves the performance of our instrument. Combining the superior beam-steering capability of the EODs, force acquisition via back-focal-plane interferometry, and dual high-speed FPGA-based feedback loops, we apply precise and constant loads to study the dynamics of interactions between actin and myosin. The same concept applies to studies of other biomolecular interactions.
DOI
Optical manipulation of nematic colloids at the interfaces in azo-dye-doped liquid crystals
Andy Ying Guey Fuh, Ming-Hsien Li, Te-Wei Chang, Yu-I Lee, and Shing Trong Wu
This study demonstrates the optical manipulation of colloids dispersed in azo-dye-doped liquid crystals (DDLCs) where the accumulation occurs at the interfaces of the phase domains. We explain the mechanism related to the formation of the domains and the movement of the colloids in DDLCs with respect to the isomerization of azo-dye molecules via the illumination of laser beams. The colloids are dragged to the interfaces of the isotropic/nematic domain and the air bubble/isotropic domain by molecular interaction and Marangoni flows.
DOI
This study demonstrates the optical manipulation of colloids dispersed in azo-dye-doped liquid crystals (DDLCs) where the accumulation occurs at the interfaces of the phase domains. We explain the mechanism related to the formation of the domains and the movement of the colloids in DDLCs with respect to the isomerization of azo-dye molecules via the illumination of laser beams. The colloids are dragged to the interfaces of the isotropic/nematic domain and the air bubble/isotropic domain by molecular interaction and Marangoni flows.
DOI
Estimation of actomyosin active force maintained by tropomyosin and troponin complex under vertical forces in the in vitro motility assay system
Shuya Ishii, Masataka Kawai, Shin'ichi Ishiwata, Madoka Suzuki
The interaction between actin filaments and myosin molecular motors is a power source of a variety of cellular functions including cell division, cell motility, and muscular contraction. In vitro motility assay examines actin filaments interacting with myosin molecules that are adhered to a substrate (e.g., glass surface). This assay has been the standard method of studying the molecular mechanisms of contraction under an optical microscope. While the force generation has been measured through an optically trapped bead to which an actin filament is attached, a force vector vertical to the glass surface has been largely ignored with the in vitro motility assay. The vertical vector is created by the gap (distance) between the trapped bead and the glass surface. In this report, we propose a method to estimate the angle between the actin filament and the glass surface by optically determining the gap size. This determination requires a motorized stage in a standard epi-fluorescence microscope equipped with optical tweezers. This facile method is applied to force measurements using both pure actin filaments, and thin filaments reconstituted from actin, tropomyosin and troponin. We find that the angle-corrected force per unit filament length in the active condition (pCa = 5.0) decreases as the angle between the filament and the glass surface increases; i.e. as the force in the vertical direction increases. At the same time, we demonstrate that the force on reconstituted thin filaments is approximately 1.5 times larger than that on pure actin filaments. The range of angles we tested was between 11° and 36° with the estimated measurement error less than 6°. These results suggest the ability of cytoplasmic tropomyosin isoforms maintaining actomyosin active force to stabilize cytoskeletal architecture.
DOI
The interaction between actin filaments and myosin molecular motors is a power source of a variety of cellular functions including cell division, cell motility, and muscular contraction. In vitro motility assay examines actin filaments interacting with myosin molecules that are adhered to a substrate (e.g., glass surface). This assay has been the standard method of studying the molecular mechanisms of contraction under an optical microscope. While the force generation has been measured through an optically trapped bead to which an actin filament is attached, a force vector vertical to the glass surface has been largely ignored with the in vitro motility assay. The vertical vector is created by the gap (distance) between the trapped bead and the glass surface. In this report, we propose a method to estimate the angle between the actin filament and the glass surface by optically determining the gap size. This determination requires a motorized stage in a standard epi-fluorescence microscope equipped with optical tweezers. This facile method is applied to force measurements using both pure actin filaments, and thin filaments reconstituted from actin, tropomyosin and troponin. We find that the angle-corrected force per unit filament length in the active condition (pCa = 5.0) decreases as the angle between the filament and the glass surface increases; i.e. as the force in the vertical direction increases. At the same time, we demonstrate that the force on reconstituted thin filaments is approximately 1.5 times larger than that on pure actin filaments. The range of angles we tested was between 11° and 36° with the estimated measurement error less than 6°. These results suggest the ability of cytoplasmic tropomyosin isoforms maintaining actomyosin active force to stabilize cytoskeletal architecture.
DOI
Theoretical investigation on asymmetrical spinning and orbiting motions of particles in a tightly focused power-exponent azimuthal-variant vector field
Yingdi Zhang, Yuxiong Xue, Zhuqing Zhu, Guanghao Rui, Yiping Cui, and Bing Gu
We generate a new kind of azimuthal-variant vector field with a distribution of states of polarization (SoPs) described by the square of the azimuthal angle. Owing to asymmetrical SoPs distribution of this localized linearly polarized vector field, the tightly focused field exhibits a double half-moon shaped pattern with the localized elliptical polarization in the cross section of field at the focal plane. Moreover, we study the three-dimensional distributions of spin and orbital linear and angular momenta in the focal region. We numerically investigate the gradient force, radiation force, spin torque, and orbital torque on a dielectric Rayleigh particle produced by the tightly focused vector field. It is found that asymmetrical spinning and orbiting motions of trapped Rayleigh particles can be realized by the use of a tight vector field with power-exponent azimuthal-variant SoPs.
DOI
We generate a new kind of azimuthal-variant vector field with a distribution of states of polarization (SoPs) described by the square of the azimuthal angle. Owing to asymmetrical SoPs distribution of this localized linearly polarized vector field, the tightly focused field exhibits a double half-moon shaped pattern with the localized elliptical polarization in the cross section of field at the focal plane. Moreover, we study the three-dimensional distributions of spin and orbital linear and angular momenta in the focal region. We numerically investigate the gradient force, radiation force, spin torque, and orbital torque on a dielectric Rayleigh particle produced by the tightly focused vector field. It is found that asymmetrical spinning and orbiting motions of trapped Rayleigh particles can be realized by the use of a tight vector field with power-exponent azimuthal-variant SoPs.
DOI
Fundamental Limits of Optical Tweezer Nanoparticle Manipulation Speeds
Jeffrey E. Melzer and Euan McLeod
Optical tweezers are a noncontact method of 3D positioning applicable to the fields of micro- and nanomanipulation and assembly, among others. In these applications, the ability to manipulate particles over relatively long distances at high speed is essential in determining overall process efficiency and throughput. In order to maximize manipulation speeds, it is necessary to increase the trapping laser power, which is often accompanied by undesirable heating effects due to material absorption. As such, the majority of previous studies focus primarily on trapping large dielectric microspheres using slow movement speeds at low laser powers, over relatively short translation distances. In contrast, we push nanoparticle manipulation beyond the region in which maximum lateral movement speed is linearly proportional to laser power, and investigate the fundamental limits imposed by material absorption, thus quantifying maximum possible speeds attainable with optical tweezers. We find that gold and silver nanospheres of diameter 100 nm are limited to manipulation speeds of ∼0.15 mm/s, while polystyrene spheres of diameter 160 nm can reach speeds up to ∼0.17 mm/s, over distances ranging from 0.1 to 1 mm. When the laser power is increased beyond the values used for these maximum manipulation speeds, the nanoparticles are no longer stably trapped in 3D due to weak confinement as a result of material absorption, heating, microbubble formation, and enhanced Brownian motion. We compared this result to our theoretical model, incorporating optical forces in the Rayleigh regime, Stokes’ drag, and absorption effects, and found good agreement. These results show that optical tweezers can be fast enough to compete with other common, serial rapid prototyping and nanofabrication approaches.
Optical tweezers are a noncontact method of 3D positioning applicable to the fields of micro- and nanomanipulation and assembly, among others. In these applications, the ability to manipulate particles over relatively long distances at high speed is essential in determining overall process efficiency and throughput. In order to maximize manipulation speeds, it is necessary to increase the trapping laser power, which is often accompanied by undesirable heating effects due to material absorption. As such, the majority of previous studies focus primarily on trapping large dielectric microspheres using slow movement speeds at low laser powers, over relatively short translation distances. In contrast, we push nanoparticle manipulation beyond the region in which maximum lateral movement speed is linearly proportional to laser power, and investigate the fundamental limits imposed by material absorption, thus quantifying maximum possible speeds attainable with optical tweezers. We find that gold and silver nanospheres of diameter 100 nm are limited to manipulation speeds of ∼0.15 mm/s, while polystyrene spheres of diameter 160 nm can reach speeds up to ∼0.17 mm/s, over distances ranging from 0.1 to 1 mm. When the laser power is increased beyond the values used for these maximum manipulation speeds, the nanoparticles are no longer stably trapped in 3D due to weak confinement as a result of material absorption, heating, microbubble formation, and enhanced Brownian motion. We compared this result to our theoretical model, incorporating optical forces in the Rayleigh regime, Stokes’ drag, and absorption effects, and found good agreement. These results show that optical tweezers can be fast enough to compete with other common, serial rapid prototyping and nanofabrication approaches.
Microscopic Engine Powered by Critical Demixing
Falko Schmidt, Alessandro Magazzù, Agnese Callegari, Luca Biancofiore, Frank Cichos, and Giovanni Volpe
We experimentally demonstrate a microscopic engine powered by the local reversible demixing of a critical mixture. We show that, when an absorbing microsphere is optically trapped by a focused laser beam in a subcritical mixture, it is set into rotation around the optical axis of the beam because of the emergence of diffusiophoretic propulsion. This behavior can be controlled by adjusting the optical power, the temperature, and the criticality of the mixture.
DOI
We experimentally demonstrate a microscopic engine powered by the local reversible demixing of a critical mixture. We show that, when an absorbing microsphere is optically trapped by a focused laser beam in a subcritical mixture, it is set into rotation around the optical axis of the beam because of the emergence of diffusiophoretic propulsion. This behavior can be controlled by adjusting the optical power, the temperature, and the criticality of the mixture.
DOI
Switching of Giant Lateral Force on Sub‐10 nm Particle Using Phase‐Change Nanoantenna
Tun Cao, Jiaxin Bao, Libang Mao
We numerically show that a giant lateral optical force (LOF) acting on sub‐10 nm non‐chiral particles can be obtained using a dipole‐quadrupole (DQ) Fano resonance (FR). This DQ‐FR is excited by an asymmetric plasmonic bowtie nanoantenna array (BNA), which is based on a Au/Ge2Sb2Te5/Au trilayer. The LOF behaves in a direction in which the incident light has neither a spin‐orbit coupling nor any wave propagation. Analytical theory reveals that the LOF originates from the “hotspot” established by the DQ‐FR in the asymmetric BNA. The direction of DQ‐FR induced LOF is reversibly switched with the phase transition of Ge2Sb2Te5, which in turn pushes the achiral nanoparticle sideways in the opposite direction. A photo thermal model is used to study the temporal variation of the temperature of the Ge2Sb2Te5 film to show the potential for transiting the Ge2Sb2Te5 phase. Particularly, the design of hybrid nanostructure integrating a ground metal mirror can excite a strong magnetic resonance, which enhances the DQ‐FR induced LOF and reduces the Brownian motion effect on the nanoparticles. Hence, our scheme leads to a rapid and stable transportation of nanoparticles with radii as small as 10 nm.
DOI
We numerically show that a giant lateral optical force (LOF) acting on sub‐10 nm non‐chiral particles can be obtained using a dipole‐quadrupole (DQ) Fano resonance (FR). This DQ‐FR is excited by an asymmetric plasmonic bowtie nanoantenna array (BNA), which is based on a Au/Ge2Sb2Te5/Au trilayer. The LOF behaves in a direction in which the incident light has neither a spin‐orbit coupling nor any wave propagation. Analytical theory reveals that the LOF originates from the “hotspot” established by the DQ‐FR in the asymmetric BNA. The direction of DQ‐FR induced LOF is reversibly switched with the phase transition of Ge2Sb2Te5, which in turn pushes the achiral nanoparticle sideways in the opposite direction. A photo thermal model is used to study the temporal variation of the temperature of the Ge2Sb2Te5 film to show the potential for transiting the Ge2Sb2Te5 phase. Particularly, the design of hybrid nanostructure integrating a ground metal mirror can excite a strong magnetic resonance, which enhances the DQ‐FR induced LOF and reduces the Brownian motion effect on the nanoparticles. Hence, our scheme leads to a rapid and stable transportation of nanoparticles with radii as small as 10 nm.
DOI
Tuesday, April 17, 2018
Sprouting angiogenesis induces significant mechanical heterogeneities and ECM stiffening across length scales in fibrin hydrogels
Benjamin A. Juliar, Mark T. Keating, Yen P. Kong, Elliot L. Botvinick, Andrew J.Putnam
Matrix stiffness is a well-established instructive cue in two-dimensional cell cultures. Its roles in morphogenesis in 3-dimensional (3D) cultures, and the converse effects of cells on the mechanics of their surrounding microenvironment, have been more elusive given the absence of suitable methods to quantify stiffness on a length-scale relevant for individual cell-extracellular matrix (ECM) interactions. In this study, we applied traditional bulk rheology and laser tweezers-based active microrheology to probe mechanics across length scales during the complex multicellular process of capillary morphogenesis in 3D, and further characterized the relative contributions of neovessels and supportive stromal cells to dynamic changes in stiffness over time. Our data show local ECM stiffness was highly heterogeneous around sprouting capillaries, and the variation progressively increased with time. Both endothelial cells and stromal support cells progressively stiffened the ECM, with the changes in bulk properties dominated by the latter. Interestingly, regions with high micro-stiffness did not necessarily correlate with remodeled regions of high ECM density as shown by confocal reflectance microscopy. Collectively, these findings, especially the large spatiotemporal variations in local stiffness around cells during morphogenesis in soft 3D fibrin gels, underscore that characterizing ECM mechanics across length scales. provides an opportunity to attain a deeper mechanobiological understanding of the microenvironment's roles in cell fate and tissue patterning.
DOI
Matrix stiffness is a well-established instructive cue in two-dimensional cell cultures. Its roles in morphogenesis in 3-dimensional (3D) cultures, and the converse effects of cells on the mechanics of their surrounding microenvironment, have been more elusive given the absence of suitable methods to quantify stiffness on a length-scale relevant for individual cell-extracellular matrix (ECM) interactions. In this study, we applied traditional bulk rheology and laser tweezers-based active microrheology to probe mechanics across length scales during the complex multicellular process of capillary morphogenesis in 3D, and further characterized the relative contributions of neovessels and supportive stromal cells to dynamic changes in stiffness over time. Our data show local ECM stiffness was highly heterogeneous around sprouting capillaries, and the variation progressively increased with time. Both endothelial cells and stromal support cells progressively stiffened the ECM, with the changes in bulk properties dominated by the latter. Interestingly, regions with high micro-stiffness did not necessarily correlate with remodeled regions of high ECM density as shown by confocal reflectance microscopy. Collectively, these findings, especially the large spatiotemporal variations in local stiffness around cells during morphogenesis in soft 3D fibrin gels, underscore that characterizing ECM mechanics across length scales. provides an opportunity to attain a deeper mechanobiological understanding of the microenvironment's roles in cell fate and tissue patterning.
DOI
Tunable optical forces enhanced by plasmonic modes hybridization in optical trapping of gold nanorods with plasmonic nanocavity
Wen-Hao Huang, Shun-Feng Li, Hai-Tao Xu, Zheng-Xun Xiang, Yong-Bing Long, and Hai-Dong Deng
The optomechanical interaction between a plasmonic nanocavity and a gold nanorod through optical forces is demonstrated. It is revealed that strong localized plasmon resonance mode hybridization induced by a gold nanorod results in the resonance mode of the nanocavity splitting into two different plasmon resonance modes (bonding plasmon resonance mode and antibonding plasmon resonance mode). When the whole system (gold nanorod and gold nanocavity) is excited at the antibonding plasmon mode, the gold nanorod can receive an optical pushing force and be pushed away from the gold nanocavity. On the other hand, an optical pulling force acts on the gold nanorod and the gold nanorod can be trapped by the gold nanocavity when the plasmonic tweezers work at the bonding mode. The optical pulling force acting on the gold nanorod can be enhanced by two orders of magnitude larger than that of the same sized dielectric nanorod, which benefits from the strong resonant nearfield interaction between the gold nanorod and the gold nanocavity. More importantly, the shape and the position of the optical potential can be tuned by tailoring the wavelength of the laser used in the optical trapping, which can be used to manipulate the gold nanorod within a nanoscale region. Our findings have important implications for optical trapping, manipulation, sorting, and sieving of plasmonic nanoparticles with plasmonic tweezers.
DOI
The optomechanical interaction between a plasmonic nanocavity and a gold nanorod through optical forces is demonstrated. It is revealed that strong localized plasmon resonance mode hybridization induced by a gold nanorod results in the resonance mode of the nanocavity splitting into two different plasmon resonance modes (bonding plasmon resonance mode and antibonding plasmon resonance mode). When the whole system (gold nanorod and gold nanocavity) is excited at the antibonding plasmon mode, the gold nanorod can receive an optical pushing force and be pushed away from the gold nanocavity. On the other hand, an optical pulling force acts on the gold nanorod and the gold nanorod can be trapped by the gold nanocavity when the plasmonic tweezers work at the bonding mode. The optical pulling force acting on the gold nanorod can be enhanced by two orders of magnitude larger than that of the same sized dielectric nanorod, which benefits from the strong resonant nearfield interaction between the gold nanorod and the gold nanocavity. More importantly, the shape and the position of the optical potential can be tuned by tailoring the wavelength of the laser used in the optical trapping, which can be used to manipulate the gold nanorod within a nanoscale region. Our findings have important implications for optical trapping, manipulation, sorting, and sieving of plasmonic nanoparticles with plasmonic tweezers.
DOI
A cooperative transition from the semi-flexible to the flexible regime of polymer elasticity: Mitoxantrone-induced DNA condensation
C.H.M.Lima, G.O.Almeida, M.S.Rocha
We report a high cooperative transition from the semi-flexible to the flexible regime of polymer elasticity during the interaction of the DNA molecule with the chemotherapeutic drug Mitoxantrone (MTX). By using single molecule force spectroscopy, we show that the force-extension curves of the DNA-MTX complexes deviate from the typical worm-like chain behavior as the MTX concentration in the sample increases, becoming straight lines for sufficiently high drug concentrations. The behavior of the radius of gyration of the complexes as a function of the bound MTX concentration was used to quantitatively investigate the cooperativity of the condensation process. The present methodology can be promptly applied to other ligands that condense the DNA molecule upon binding, opening new possibilities in the investigation of this type of process and, more generally, in the investigation of phase transitions in polymer physics.
DOI
We report a high cooperative transition from the semi-flexible to the flexible regime of polymer elasticity during the interaction of the DNA molecule with the chemotherapeutic drug Mitoxantrone (MTX). By using single molecule force spectroscopy, we show that the force-extension curves of the DNA-MTX complexes deviate from the typical worm-like chain behavior as the MTX concentration in the sample increases, becoming straight lines for sufficiently high drug concentrations. The behavior of the radius of gyration of the complexes as a function of the bound MTX concentration was used to quantitatively investigate the cooperativity of the condensation process. The present methodology can be promptly applied to other ligands that condense the DNA molecule upon binding, opening new possibilities in the investigation of this type of process and, more generally, in the investigation of phase transitions in polymer physics.
DOI
Force feedback effects on single molecule hopping and pulling experiments
M. Rico-Pasto, I. Pastor, and F. Ritort
Single-molecule experiments with optical tweezers have become an important tool to study the properties and mechanisms of biological systems, such as cells and nucleic acids. In particular, force unzipping experiments have been used to extract the thermodynamics and kinetics of folding and unfolding reactions. In hopping experiments, a molecule executes transitions between the unfolded and folded states at a preset value of the force [constant force mode (CFM) under force feedback] or trap position [passive mode (PM) without feedback] and the force-dependent kinetic rates extracted from the lifetime of each state (CFM) and the rupture force distributions (PM) using the Bell-Evans model. However, hopping experiments in the CFM are known to overestimate molecular distances and folding free energies for fast transitions compared to the response time of the feedback. In contrast, kinetic rate measurements from pulling experiments have been mostly done in the PM while the CFM is seldom implemented in pulling protocols. Here, we carry out hopping and pulling experiments in a short DNA hairpin in the PM and CFM at three different temperatures (6 °C, 25 °C, and 45 °C) exhibiting largely varying kinetic rates. As expected, we find that equilibrium hopping experiments in the CFM and PM perform well at 6 °C (where kinetics are slow), whereas the CFM overestimates molecular parameters at 45 °C (where kinetics are fast). In contrast, nonequilibrium pulling experiments perform well in both modes at all temperatures. This demonstrates that the same kind of feedback algorithm in the CFM leads to more reliable determination of the folding reaction parameters in irreversible pulling experiments.
DOI
Single-molecule experiments with optical tweezers have become an important tool to study the properties and mechanisms of biological systems, such as cells and nucleic acids. In particular, force unzipping experiments have been used to extract the thermodynamics and kinetics of folding and unfolding reactions. In hopping experiments, a molecule executes transitions between the unfolded and folded states at a preset value of the force [constant force mode (CFM) under force feedback] or trap position [passive mode (PM) without feedback] and the force-dependent kinetic rates extracted from the lifetime of each state (CFM) and the rupture force distributions (PM) using the Bell-Evans model. However, hopping experiments in the CFM are known to overestimate molecular distances and folding free energies for fast transitions compared to the response time of the feedback. In contrast, kinetic rate measurements from pulling experiments have been mostly done in the PM while the CFM is seldom implemented in pulling protocols. Here, we carry out hopping and pulling experiments in a short DNA hairpin in the PM and CFM at three different temperatures (6 °C, 25 °C, and 45 °C) exhibiting largely varying kinetic rates. As expected, we find that equilibrium hopping experiments in the CFM and PM perform well at 6 °C (where kinetics are slow), whereas the CFM overestimates molecular parameters at 45 °C (where kinetics are fast). In contrast, nonequilibrium pulling experiments perform well in both modes at all temperatures. This demonstrates that the same kind of feedback algorithm in the CFM leads to more reliable determination of the folding reaction parameters in irreversible pulling experiments.
DOI
Analysis of lateral binding force exerted on a bi-sphere induced by an elliptic Gaussian beam
Jing Bai, Zhen-Sen Wu, Cheng-Xian Ge, Zheng-Jun Li, Tan Qu, and Qing-Chao Shang
Based on the generalized Lorentz–Mie theory (GLMT) and the localized approximation of the beam shape coefficients, we derived the expansions of incident elliptic Gaussian (EG) beams in terms of spherical vector wave functions (SVWFs). Utilizing multiple scattering (MS) equations and electromagnetic momentum (EM) theory, the lateral binding force (BF) exerted on a bi-sphere induced by an EG beam is calculated. Numerical effects of various parameters such as beam waist widths, beam polarization states, incident wavelengths, particle sizes, and material losses are analyzed and compared with the results of a circular Gaussian (CG) beam in detail. The observed dependence of the separation of optically bound particles on the incidence of an EG beam is in agreement with earlier theoretical predictions. Accurate investigation of BF induced by an EG beam could provide an effective test for further research on BF between more complex particles, which plays an important role in using optical manipulation on particle self-assembly.
DOI
Based on the generalized Lorentz–Mie theory (GLMT) and the localized approximation of the beam shape coefficients, we derived the expansions of incident elliptic Gaussian (EG) beams in terms of spherical vector wave functions (SVWFs). Utilizing multiple scattering (MS) equations and electromagnetic momentum (EM) theory, the lateral binding force (BF) exerted on a bi-sphere induced by an EG beam is calculated. Numerical effects of various parameters such as beam waist widths, beam polarization states, incident wavelengths, particle sizes, and material losses are analyzed and compared with the results of a circular Gaussian (CG) beam in detail. The observed dependence of the separation of optically bound particles on the incidence of an EG beam is in agreement with earlier theoretical predictions. Accurate investigation of BF induced by an EG beam could provide an effective test for further research on BF between more complex particles, which plays an important role in using optical manipulation on particle self-assembly.
DOI
Application of a near-infrared laser tweezers Raman spectroscopy system for label-free analysis and differentiation of diabetic red blood cells
Jinyong Lin, Lingdong Shao, Sufang Qiu, Xingwu Huang, Mengmeng Liu, Zuci Zheng, Duo Lin, Yongliang Xu, Zhihua Li, Yao Lin, Rong Chen, and Shangyuan Feng
A home-made near-infrared laser tweezers Raman spectroscopy (LTRS) system was applied to detect hemoglobin variation in red blood cells (RBCs) from diabetes without exogenous labeling. Results showed significant spectral differences existed between the diabetic and normal RBCs, including the peaks dominated by protein components (e.g. 1003 cm−1) and heme groups (e.g. 753 cm−1) in RBCs, and accurate classification results for diabetes detection were obtained by linear discriminant analysis with 100% sensitivity (i.e. no false negatives in the study). This work indicated the great promise of LTRS as a label-free RBC analytical tool for improving the accurate detection of type II diabetes.
DOI
A home-made near-infrared laser tweezers Raman spectroscopy (LTRS) system was applied to detect hemoglobin variation in red blood cells (RBCs) from diabetes without exogenous labeling. Results showed significant spectral differences existed between the diabetic and normal RBCs, including the peaks dominated by protein components (e.g. 1003 cm−1) and heme groups (e.g. 753 cm−1) in RBCs, and accurate classification results for diabetes detection were obtained by linear discriminant analysis with 100% sensitivity (i.e. no false negatives in the study). This work indicated the great promise of LTRS as a label-free RBC analytical tool for improving the accurate detection of type II diabetes.
DOI
Numerical study on trapping and guiding of nanoparticles in a flow using scattering field of laser light
Naomichi Yokoi, Yoshihisa Aizu
Optical trapping and guiding using laser have been proven to be useful for non-contact and non-invasive manipulation of small objects such as biological cells, organelles within cells, and dielectric particles. We have numerically investigated so far the motion of a Brownian particle suspended in still water under the illumination of a speckle pattern generated by the interference of coherent light scattered by a rough object. In the present study, we investigate numerically the motion of a particle in a water flow under the illumination of a speckle pattern that is at rest or in motion. Trajectory of the particle is simulated in relation with its size, flow velocity, maximum irradiance, and moving velocity of the speckle pattern to confirm the feasibility of the present method for performing optical trapping and guiding of the particle in the flow.
DOI
Optical trapping and guiding using laser have been proven to be useful for non-contact and non-invasive manipulation of small objects such as biological cells, organelles within cells, and dielectric particles. We have numerically investigated so far the motion of a Brownian particle suspended in still water under the illumination of a speckle pattern generated by the interference of coherent light scattered by a rough object. In the present study, we investigate numerically the motion of a particle in a water flow under the illumination of a speckle pattern that is at rest or in motion. Trajectory of the particle is simulated in relation with its size, flow velocity, maximum irradiance, and moving velocity of the speckle pattern to confirm the feasibility of the present method for performing optical trapping and guiding of the particle in the flow.
DOI
Thursday, April 12, 2018
Probing Spatiotemporal Stability of Optical Matter by Polarization Modulation
Fan Nan and Zijie Yan
Light-driven self-organization of plasmonic nanoparticles via optical binding interactions offers a unique route to assemble mesoscale photonic clusters and chains. However, stability becomes an issue when more nanoparticles are added into the clusters and chains, since the theoretical optical binding strength is inhomogeneous and anisotropic in optical matter systems. Here we study the spatiotemporal stability of optical matter chains self-organized by two to eight ultrauniform gold nanospheres in a linearly polarized optical line trap. Perturbations are introduced into the nanosphere chains by periodically switching the polarization to be either parallel or perpendicular to the orientation of the chains, where the spatial and temporal variation of optical binding strength has been revealed. In addition, we found that the average oscillation amplitude and stability of the particles can be tuned by the frequency of polarization modulation. These results demonstrate a new way to study and improve the stability of optical matter and provide a promising strategy in engineering optical forces at the mesoscale.
DOI
Light-driven self-organization of plasmonic nanoparticles via optical binding interactions offers a unique route to assemble mesoscale photonic clusters and chains. However, stability becomes an issue when more nanoparticles are added into the clusters and chains, since the theoretical optical binding strength is inhomogeneous and anisotropic in optical matter systems. Here we study the spatiotemporal stability of optical matter chains self-organized by two to eight ultrauniform gold nanospheres in a linearly polarized optical line trap. Perturbations are introduced into the nanosphere chains by periodically switching the polarization to be either parallel or perpendicular to the orientation of the chains, where the spatial and temporal variation of optical binding strength has been revealed. In addition, we found that the average oscillation amplitude and stability of the particles can be tuned by the frequency of polarization modulation. These results demonstrate a new way to study and improve the stability of optical matter and provide a promising strategy in engineering optical forces at the mesoscale.
DOI
Measuring Microtubule Supertwist and Defects by Three-Dimensional-Force-Clamp Tracking of Single Kinesin-1 Motors
Michael Bugiel, Aniruddha Mitra, Salvatore Girardo, Stefan Diez, and Erik Schäffer
Three-dimensional (3D) nanometer tracking of single biomolecules provides important information about their biological function. However, existing microscopy approaches often have only limited spatial or temporal precision and do not allow the application of defined loads. Here, we developed and applied a high-precision 3D-optical-tweezers force clamp to track in vitro the 3D motion of single kinesin-1 motor proteins along microtubules. To provide the motors with unimpeded access to the whole microtubule lattice, we mounted the microtubules on topographic surface features generated by UV-nanoimprint lithography. Because kinesin-1 motors processively move along individual protofilaments, we could determine the number of protofilaments the microtubules were composed of by measuring the helical pitches of motor movement on supertwisted microtubules. Moreover, we were able to identify defects in microtubules, most likely arising from local changes in the protofilament number. While it is hypothesized that microtubule supertwist and defects can severely influence the function of motors and other microtubule-associated proteins, the presented method allows for the first time to fully map the microtubule lattice in situ. This mapping allows the correlation of motor-filament interactions with the microtubule fine-structure. With the additional ability to apply loads, we expect our 3D-optical-tweezers force clamp to become a valuable tool for obtaining a wide range of information from other biological systems, inaccessible by two-dimensional and/or ensemble measurements.
DOI
Three-dimensional (3D) nanometer tracking of single biomolecules provides important information about their biological function. However, existing microscopy approaches often have only limited spatial or temporal precision and do not allow the application of defined loads. Here, we developed and applied a high-precision 3D-optical-tweezers force clamp to track in vitro the 3D motion of single kinesin-1 motor proteins along microtubules. To provide the motors with unimpeded access to the whole microtubule lattice, we mounted the microtubules on topographic surface features generated by UV-nanoimprint lithography. Because kinesin-1 motors processively move along individual protofilaments, we could determine the number of protofilaments the microtubules were composed of by measuring the helical pitches of motor movement on supertwisted microtubules. Moreover, we were able to identify defects in microtubules, most likely arising from local changes in the protofilament number. While it is hypothesized that microtubule supertwist and defects can severely influence the function of motors and other microtubule-associated proteins, the presented method allows for the first time to fully map the microtubule lattice in situ. This mapping allows the correlation of motor-filament interactions with the microtubule fine-structure. With the additional ability to apply loads, we expect our 3D-optical-tweezers force clamp to become a valuable tool for obtaining a wide range of information from other biological systems, inaccessible by two-dimensional and/or ensemble measurements.
DOI
Dynamic analysis and rotation experiment of an optical-trapped microsphere in air
Wenqiang Li, Nan Li, Yu Shen, Zhenhai Fu, Heming Su, and Huizhu Hu
A dual-fiber optical trap system to trap and rotate a borosilicate microsphere has been proposed and experimentally demonstrated. The trapping system can be used as a probe to measure environmental parameters, such as torque, force, and viscosity of the surrounding medium. Under various conditions with different fiber misalignments, optical power, and fiber separation, the trapped sphere will exhibit three motion profiles including random oscillation, round rotation, and abnormal rotation. The power spectrum analysis method is used to measure rotation rates up to 385 Hz, which can be further increased by increasing laser power. In addition, simulation and experiment show consistent results in rotation rates and motion trajectory, which verifies the validity and accuracy of dynamic analysis.
DOI
A dual-fiber optical trap system to trap and rotate a borosilicate microsphere has been proposed and experimentally demonstrated. The trapping system can be used as a probe to measure environmental parameters, such as torque, force, and viscosity of the surrounding medium. Under various conditions with different fiber misalignments, optical power, and fiber separation, the trapped sphere will exhibit three motion profiles including random oscillation, round rotation, and abnormal rotation. The power spectrum analysis method is used to measure rotation rates up to 385 Hz, which can be further increased by increasing laser power. In addition, simulation and experiment show consistent results in rotation rates and motion trajectory, which verifies the validity and accuracy of dynamic analysis.
DOI
Adjustable vector Airy light-sheet single optical tweezers: negative radiation forces on a subwavelength spheroid and spin torque reversal
Farid G. Mitri
Generalized solutions of vector Airy light-sheets, adjustable per their derivative order m, are introduced stemming from the Lorenz gauge condition and Maxwell’s equations using the angular spectrum decomposition method. The Cartesian components of the incident radiated electric, magnetic and time-averaged Poynting vector fields in free space (excluding evanescent waves) are determined and computed with particular emphasis on the derivative order of the Airy light-sheet and the polarization on the magnetic vector potential forming the beam. Negative transverse time-averaged Poynting vector components can arise, while the longitudinal counterparts are always positive. Moreover, the analysis is extended to compute the optical radiation force and spin torque vector components on a lossless dielectric prolate subwavelength spheroid in the framework of the electric dipole approximation. The results show that negative forces and spin torques sign reversal arise depending on the derivative order of the beam, the polarization of the magnetic vector potential, and the orientation of the subwavelength prolate spheroid in space. The spin torque sign reversal suggests that counter-clockwise or clockwise rotations around the center of mass of the subwavelength spheroid can occur. The results find useful applications in single Airy light-sheet tweezers, particle manipulation, handling, and rotation applications to name a few examples.
DOI
Generalized solutions of vector Airy light-sheets, adjustable per their derivative order m, are introduced stemming from the Lorenz gauge condition and Maxwell’s equations using the angular spectrum decomposition method. The Cartesian components of the incident radiated electric, magnetic and time-averaged Poynting vector fields in free space (excluding evanescent waves) are determined and computed with particular emphasis on the derivative order of the Airy light-sheet and the polarization on the magnetic vector potential forming the beam. Negative transverse time-averaged Poynting vector components can arise, while the longitudinal counterparts are always positive. Moreover, the analysis is extended to compute the optical radiation force and spin torque vector components on a lossless dielectric prolate subwavelength spheroid in the framework of the electric dipole approximation. The results show that negative forces and spin torques sign reversal arise depending on the derivative order of the beam, the polarization of the magnetic vector potential, and the orientation of the subwavelength prolate spheroid in space. The spin torque sign reversal suggests that counter-clockwise or clockwise rotations around the center of mass of the subwavelength spheroid can occur. The results find useful applications in single Airy light-sheet tweezers, particle manipulation, handling, and rotation applications to name a few examples.
DOI
Advances in colloidal manipulation and transport via hydrodynamic interactions
F. Martínez-Pedrero, P. Tierno
In this review article, we highlight many recent advances in the field of micromanipulation of colloidal particles using hydrodynamic interactions (HIs), namely solvent mediated long-range interactions. At the micrsocale, the hydrodynamic laws are time reversible and the flow becomes laminar, features that allow precise manipulation and control of colloidal matter. We focus on different strategies where externally operated microstructures generate local flow fields that induce the advection and motion of the surrounding components. In addition, we review cases where the induced flow gives rise to hydrodynamic bound states that may synchronize during the process, a phenomenon essential in different systems such as those that exhibit self-assembly and swarming.
DOI
In this review article, we highlight many recent advances in the field of micromanipulation of colloidal particles using hydrodynamic interactions (HIs), namely solvent mediated long-range interactions. At the micrsocale, the hydrodynamic laws are time reversible and the flow becomes laminar, features that allow precise manipulation and control of colloidal matter. We focus on different strategies where externally operated microstructures generate local flow fields that induce the advection and motion of the surrounding components. In addition, we review cases where the induced flow gives rise to hydrodynamic bound states that may synchronize during the process, a phenomenon essential in different systems such as those that exhibit self-assembly and swarming.
DOI
Nanoscale orbital angular momentum beam instabilities in engineered nonlinear colloidal media
Jingbo Sun, Salih Z. Silahli, Wiktor Walasik, Qi Li, Eric Johnson, and Natalia M. Litchinitser
Colloidal media with well-defined optical properties have been widely used as model systems in many fundamental and applied studies of light-matter interactions in complex media. Recent progress in the field of engineered nanoscale optical materials with fundamentally new physical properties opens new opportunities for tailoring the properties of colloids. In this work, we experimentally demonstrate the evolution of the optical vortex beams of different topological charges propagating in engineered nano-colloidal suspension of negative polarizability with saturable nonlinearities. Due to the high power of the incident beam, the modulation instability leads to an exponential growth of weak perturbations and thus splits the original vortex beam into a necklace beam consisting of several bright spots. At a fixed power, the number of observed bright spots is intrinsically determined by the topological charge of the incident beam and agrees well with the predictions of our linear stability analysis and numerical simulations. Besides contributing to the fundamental science of light-matter interactions in engineered soft-matter media, this work opens new opportunities for dynamic optical manipulation and transmission of light through scattering media as well as formation of complex optical patterns and light filamentation in naturally existing colloids such as fog and clouds.
DOI
Colloidal media with well-defined optical properties have been widely used as model systems in many fundamental and applied studies of light-matter interactions in complex media. Recent progress in the field of engineered nanoscale optical materials with fundamentally new physical properties opens new opportunities for tailoring the properties of colloids. In this work, we experimentally demonstrate the evolution of the optical vortex beams of different topological charges propagating in engineered nano-colloidal suspension of negative polarizability with saturable nonlinearities. Due to the high power of the incident beam, the modulation instability leads to an exponential growth of weak perturbations and thus splits the original vortex beam into a necklace beam consisting of several bright spots. At a fixed power, the number of observed bright spots is intrinsically determined by the topological charge of the incident beam and agrees well with the predictions of our linear stability analysis and numerical simulations. Besides contributing to the fundamental science of light-matter interactions in engineered soft-matter media, this work opens new opportunities for dynamic optical manipulation and transmission of light through scattering media as well as formation of complex optical patterns and light filamentation in naturally existing colloids such as fog and clouds.
DOI
Wednesday, April 11, 2018
Escape from an Optoelectronic Tweezer Trap: experimental results and simulations
Shuailong Zhang, Adele Nikitina, Yujie Chen, Yanfeng Zhang, Lin Liu, Andrew G. Flood, Joan Juvert, M. Dean Chamberlain, Nazir P. Kherani, Steven L. Neale, and Aaron R. Wheeler
Optoelectronic tweezers (OET) are a microsystem actuation technology capable of moving microparticles at mm s−1 velocities with nN forces. In this work, we analyze the behavior of particles manipulated by negative dielectrophoresis (DEP) forces in an OET trap. A user-friendly computer interface was developed to generate a circular rotating light pattern to control the movement of the particles, allowing their force profiles to be conveniently measured. Three-dimensional simulations were carried out to clarify the experimental results, and the DEP forces acting on the particles were simulated by integrating the Maxwell stress tensor. The simulations matched the experimental results and enabled the determination of a new “hopping” mechanism for particle-escape from the trap. As indicated by the simulations, there exists a vertical DEP force at the edge of the light pattern that pushes up particles to a region with a smaller horizontal DEP force. We propose that this phenomenon will be important to consider for the design of OET micromanipulation experiments for a wide range of applications.
DOI
Optoelectronic tweezers (OET) are a microsystem actuation technology capable of moving microparticles at mm s−1 velocities with nN forces. In this work, we analyze the behavior of particles manipulated by negative dielectrophoresis (DEP) forces in an OET trap. A user-friendly computer interface was developed to generate a circular rotating light pattern to control the movement of the particles, allowing their force profiles to be conveniently measured. Three-dimensional simulations were carried out to clarify the experimental results, and the DEP forces acting on the particles were simulated by integrating the Maxwell stress tensor. The simulations matched the experimental results and enabled the determination of a new “hopping” mechanism for particle-escape from the trap. As indicated by the simulations, there exists a vertical DEP force at the edge of the light pattern that pushes up particles to a region with a smaller horizontal DEP force. We propose that this phenomenon will be important to consider for the design of OET micromanipulation experiments for a wide range of applications.
DOI
Atorvastatin treatment softens human red blood cells: an optical tweezers study
Vahid Sheikh-Hasani, Mehrad Babaei, Ali Azadbakht, Hamidreza Pazoki-Toroudi, Alireza Mashaghi, Ali Akbar Moosavi-Movahedi, and Seyed Nader Seyed Reihani
Optical tweezers are proven indispensable single-cell micro-manipulation and mechanical phenotyping tools. In this study, we have used optical tweezers for measuring the viscoelastic properties of human red blood cells (RBCs). Comparison of the viscoelastic features of the healthy fresh and atorvastatin treated cells revealed that the drug softens the cells. Using a simple modeling approach, we proposed a molecular model that explains the drug-induced softening of the RBC membrane. Our results suggest that direct interactions between the drug and cytoskeletal components underlie the drug-induced softening of the cells.
DOI
Optical tweezers are proven indispensable single-cell micro-manipulation and mechanical phenotyping tools. In this study, we have used optical tweezers for measuring the viscoelastic properties of human red blood cells (RBCs). Comparison of the viscoelastic features of the healthy fresh and atorvastatin treated cells revealed that the drug softens the cells. Using a simple modeling approach, we proposed a molecular model that explains the drug-induced softening of the RBC membrane. Our results suggest that direct interactions between the drug and cytoskeletal components underlie the drug-induced softening of the cells.
DOI
Control over phase separation and nucleation using a laser-tweezing potential
Finlay Walton & Klaas Wynne
Control over the nucleation of new phases is highly desirable but elusive. Even though there is a long history of crystallization engineering by varying physicochemical parameters, controlling which polymorph crystallizes or whether a molecule crystallizes or forms an amorphous precipitate is still a poorly understood practice. Although there are now numerous examples of control using laser-induced nucleation, the absence of physical understanding is preventing progress. Here we show that the proximity of a liquid–liquid critical point or the corresponding binodal line can be used by a laser-tweezing potential to induce concentration gradients. A simple theoretical model shows that the stored electromagnetic energy of the laser beam produces a free-energy potential that forces phase separation or triggers the nucleation of a new phase. Experiments in a liquid mixture using a low-power laser diode confirm the effect. Phase separation and nucleation using a laser-tweezing potential explains the physics behind non-photochemical laser-induced nucleation and suggests new ways of manipulating matter.
DOI
Control over the nucleation of new phases is highly desirable but elusive. Even though there is a long history of crystallization engineering by varying physicochemical parameters, controlling which polymorph crystallizes or whether a molecule crystallizes or forms an amorphous precipitate is still a poorly understood practice. Although there are now numerous examples of control using laser-induced nucleation, the absence of physical understanding is preventing progress. Here we show that the proximity of a liquid–liquid critical point or the corresponding binodal line can be used by a laser-tweezing potential to induce concentration gradients. A simple theoretical model shows that the stored electromagnetic energy of the laser beam produces a free-energy potential that forces phase separation or triggers the nucleation of a new phase. Experiments in a liquid mixture using a low-power laser diode confirm the effect. Phase separation and nucleation using a laser-tweezing potential explains the physics behind non-photochemical laser-induced nucleation and suggests new ways of manipulating matter.
DOI
Light-Controlled Swarming and Assembly of Colloidal Particles
Jianhua Zhang, Jingjing Guo, Fangzhi Mou and Jianguo Guan
Swarms and assemblies are ubiquitous in nature and they can perform complex collective behaviors and cooperative functions that they cannot accomplish individually. In response to light, some colloidal particles (CPs), including light active and passive CPs, can mimic their counterparts in nature and organize into complex structures that exhibit collective functions with remote controllability and high temporospatial precision. In this review, we firstly analyze the structural characteristics of swarms and assemblies of CPs and point out that light-controlled swarming and assembly of CPs are generally achieved by constructing light-responsive interactions between CPs. Then, we summarize in detail the recent advances in light-controlled swarming and assembly of CPs based on the interactions arisen from optical forces, photochemical reactions, photothermal effects, and photoisomerizations, as well as their potential applications. In the end, we also envision some challenges and future prospects of light-controlled swarming and assembly of CPs. With the increasing innovations in mechanisms and control strategies with easy operation, low cost, and arbitrary applicability, light-controlled swarming and assembly of CPs may be employed to manufacture programmable materials and reconfigurable robots for cooperative grasping, collective cargo transportation, and micro- and nanoengineering.
Swarms and assemblies are ubiquitous in nature and they can perform complex collective behaviors and cooperative functions that they cannot accomplish individually. In response to light, some colloidal particles (CPs), including light active and passive CPs, can mimic their counterparts in nature and organize into complex structures that exhibit collective functions with remote controllability and high temporospatial precision. In this review, we firstly analyze the structural characteristics of swarms and assemblies of CPs and point out that light-controlled swarming and assembly of CPs are generally achieved by constructing light-responsive interactions between CPs. Then, we summarize in detail the recent advances in light-controlled swarming and assembly of CPs based on the interactions arisen from optical forces, photochemical reactions, photothermal effects, and photoisomerizations, as well as their potential applications. In the end, we also envision some challenges and future prospects of light-controlled swarming and assembly of CPs. With the increasing innovations in mechanisms and control strategies with easy operation, low cost, and arbitrary applicability, light-controlled swarming and assembly of CPs may be employed to manufacture programmable materials and reconfigurable robots for cooperative grasping, collective cargo transportation, and micro- and nanoengineering.
Experimental Test of the Differential Fluctuation Theorem and a Generalized Jarzynski Equality for Arbitrary Initial States
Thai M. Hoang, Rui Pan, Jonghoon Ahn, Jaehoon Bang, H. T. Quan, and Tongcang Li
Nonequilibrium processes of small systems such as molecular machines are ubiquitous in biology, chemistry, and physics but are often challenging to comprehend. In the past two decades, several exact thermodynamic relations of nonequilibrium processes, collectively known as fluctuation theorems, have been discovered and provided critical insights. These fluctuation theorems are generalizations of the second law and can be unified by a differential fluctuation theorem. Here we perform the first experimental test of the differential fluctuation theorem using an optically levitated nanosphere in both underdamped and overdamped regimes and in both spatial and velocity spaces. We also test several theorems that can be obtained from it directly, including a generalized Jarzynski equality that is valid for arbitrary initial states, and the Hummer-Szabo relation. Our study experimentally verifies these fundamental theorems and initiates the experimental study of stochastic energetics with the instantaneous velocity measurement.
DOI
Nonequilibrium processes of small systems such as molecular machines are ubiquitous in biology, chemistry, and physics but are often challenging to comprehend. In the past two decades, several exact thermodynamic relations of nonequilibrium processes, collectively known as fluctuation theorems, have been discovered and provided critical insights. These fluctuation theorems are generalizations of the second law and can be unified by a differential fluctuation theorem. Here we perform the first experimental test of the differential fluctuation theorem using an optically levitated nanosphere in both underdamped and overdamped regimes and in both spatial and velocity spaces. We also test several theorems that can be obtained from it directly, including a generalized Jarzynski equality that is valid for arbitrary initial states, and the Hummer-Szabo relation. Our study experimentally verifies these fundamental theorems and initiates the experimental study of stochastic energetics with the instantaneous velocity measurement.
DOI
Optimal integration of wide field illumination and holographic optical tweezers for multimodal microscopy with ultimate flexibility and versatility
Sang-Hyuk Lee
We introduce one-of-a-kind optical microscope that we have developed through optimized integration of wide-field and focused-light microscopies. This new instrument has accomplished operation of the same laser for both wide field illumination and holographic focused beam illumination interchangeably or simultaneously in a way scalable to multiple lasers. We have demonstrated its powerful capability by simultaneously carrying out Epi-fluorescence, total internal reflection fluorescence microscopy, selective plane illumination microscopy, and holographic optical tweezers with five lasers. Our instrument and the optical design will provide researchers across diverse fields, cell-biology and biophysics in particular, with a practical guidance to build an all-around multimodal microscope that will further inspire the development of novel hybrid microscopy experiments.
DOI
We introduce one-of-a-kind optical microscope that we have developed through optimized integration of wide-field and focused-light microscopies. This new instrument has accomplished operation of the same laser for both wide field illumination and holographic focused beam illumination interchangeably or simultaneously in a way scalable to multiple lasers. We have demonstrated its powerful capability by simultaneously carrying out Epi-fluorescence, total internal reflection fluorescence microscopy, selective plane illumination microscopy, and holographic optical tweezers with five lasers. Our instrument and the optical design will provide researchers across diverse fields, cell-biology and biophysics in particular, with a practical guidance to build an all-around multimodal microscope that will further inspire the development of novel hybrid microscopy experiments.
DOI
Remotely controlled fusion of selected vesicles and living cells: a key issue review
Azra Bahadori, Guillermo Moreno-Pescador, Lene B Oddershede and Poul M Bendix
Remote control over fusion of single cells and vesicles has a great potential in biological and chemical research allowing both transfer of genetic material between cells and transfer of molecular content between vesicles. Membrane fusion is a critical process in biology that facilitates molecular transport and mixing of cellular cytoplasms with potential formation of hybrid cells. Cells precisely regulate internal membrane fusions with the aid of specialized fusion complexes that physically provide the energy necessary for mediating fusion. Physical factors like membrane curvature, tension and temperature, affect biological membrane fusion by lowering the associated energy barrier. This has inspired the development of physical approaches to harness the fusion process at a single cell level by using remotely controlled electromagnetic fields to trigger membrane fusion. Here, we critically review various approaches, based on lasers or electric pulses, to control fusion between individual cells or between individual lipid vesicles and discuss their potential and limitations for present and future applications within biochemistry, biology and soft matter.
DOI
Remote control over fusion of single cells and vesicles has a great potential in biological and chemical research allowing both transfer of genetic material between cells and transfer of molecular content between vesicles. Membrane fusion is a critical process in biology that facilitates molecular transport and mixing of cellular cytoplasms with potential formation of hybrid cells. Cells precisely regulate internal membrane fusions with the aid of specialized fusion complexes that physically provide the energy necessary for mediating fusion. Physical factors like membrane curvature, tension and temperature, affect biological membrane fusion by lowering the associated energy barrier. This has inspired the development of physical approaches to harness the fusion process at a single cell level by using remotely controlled electromagnetic fields to trigger membrane fusion. Here, we critically review various approaches, based on lasers or electric pulses, to control fusion between individual cells or between individual lipid vesicles and discuss their potential and limitations for present and future applications within biochemistry, biology and soft matter.
DOI
Tuesday, April 10, 2018
A remote laboratory for optical levitation of charged droplets
Daniel Galan, Oscar Isaksson, Mats Rostedt, Jonas Enger, Dag Hanstorp and Luis de la Torre
We present a remotely controlled experiment in which liquid droplets are levitated by a vertically aligned focused laser beam. The droplets levitate at the point where the photon pressure of the focused laser beam balances the gravitational force. The size of a trapped droplet can be measured by detecting the diffraction pattern created by the trapping laser light. The charge on the trapped droplet can thereafter be determined by observing its motion when a vertically directed electrical field is applied. This experiment allows a student to study many fundamental physics processes, such as photon pressure, diffraction of light, or the motion of charged particles in electrical fields. The complexity of the experiments and the concept studied make this suitable for advanced studies in physics. The laser power required in the experiment is about 1 W, which is a thousand times greater than the value of 1 mW at which lasers begin to be capable of causing harm to eyes; high voltages are also used. Further, the cost of the equipment is relatively high, which limits its availability to most undergraduate teaching laboratories. It thus constitutes an ideal experiment for remote control.
DOI
We present a remotely controlled experiment in which liquid droplets are levitated by a vertically aligned focused laser beam. The droplets levitate at the point where the photon pressure of the focused laser beam balances the gravitational force. The size of a trapped droplet can be measured by detecting the diffraction pattern created by the trapping laser light. The charge on the trapped droplet can thereafter be determined by observing its motion when a vertically directed electrical field is applied. This experiment allows a student to study many fundamental physics processes, such as photon pressure, diffraction of light, or the motion of charged particles in electrical fields. The complexity of the experiments and the concept studied make this suitable for advanced studies in physics. The laser power required in the experiment is about 1 W, which is a thousand times greater than the value of 1 mW at which lasers begin to be capable of causing harm to eyes; high voltages are also used. Further, the cost of the equipment is relatively high, which limits its availability to most undergraduate teaching laboratories. It thus constitutes an ideal experiment for remote control.
DOI
Light‐Driven Rotation of Plasmonic Nanomotors
Lei Shao, Mikael Käll
Colloidal metal nanocrystals exhibit distinct plasmonic resonances that can greatly enhance optical forces and torques. This article highlights the recent application of such particles as light‐driven rotary motors at the nanoscale. By using laser tweezers, it is possible to achieve unprecedented rotation performance in solution, providing a variety of exciting possibilities for applications ranging from nanomechanics to biochemical sensing. Recent developments in this emerging field are discussed, and the physics behind the rotation mechanism, the Brownian dynamics, and the photothermal heating effects influencing the performance of the plasmonic nanomotors are introduced. Possible applications, open questions, and interesting future developments are also discussed.
DOI
Colloidal metal nanocrystals exhibit distinct plasmonic resonances that can greatly enhance optical forces and torques. This article highlights the recent application of such particles as light‐driven rotary motors at the nanoscale. By using laser tweezers, it is possible to achieve unprecedented rotation performance in solution, providing a variety of exciting possibilities for applications ranging from nanomechanics to biochemical sensing. Recent developments in this emerging field are discussed, and the physics behind the rotation mechanism, the Brownian dynamics, and the photothermal heating effects influencing the performance of the plasmonic nanomotors are introduced. Possible applications, open questions, and interesting future developments are also discussed.
DOI
A photophoretic-trap volumetric display
D. E. Smalley, E. Nygaard, K. Squire, J. Van Wagoner, J. Rasmussen, S. Gneiting, K. Qaderi, J. Goodsell, W. Rogers, M. Lindsey, K. Costner, A. Monk, M. Pearson, B. Haymore & J. Peatross
Free-space volumetric displays, or displays that create luminous image points in space, are the technology that most closely resembles the three-dimensional displays of popular fiction1. Such displays are capable of producing images in ‘thin air’ that are visible from almost any direction and are not subject to clipping. Clipping restricts the utility of all three-dimensional displays that modulate light at a two-dimensional surface with an edge boundary; these include holographic displays, nanophotonic arrays, plasmonic displays, lenticular or lenslet displays and all technologies in which the light scattering surface and the image point are physically separate. Here we present a free-space volumetric display based on photophoretic optical trapping2 that produces full-colour graphics in free space with ten-micrometre image points using persistence of vision. This display works by first isolating a cellulose particle in a photophoretic trap created by spherical and astigmatic aberrations. The trap and particle are then scanned through a display volume while being illuminated with red, green and blue light. The result is a three-dimensional image in free space with a large colour gamut, fine detail and low apparent speckle. This platform, named the Optical Trap Display, is capable of producing image geometries that are currently unobtainable with holographic and light-field technologies, such as long-throw projections, tall sandtables and ‘wrap-around’ displays.
DOI
Free-space volumetric displays, or displays that create luminous image points in space, are the technology that most closely resembles the three-dimensional displays of popular fiction1. Such displays are capable of producing images in ‘thin air’ that are visible from almost any direction and are not subject to clipping. Clipping restricts the utility of all three-dimensional displays that modulate light at a two-dimensional surface with an edge boundary; these include holographic displays, nanophotonic arrays, plasmonic displays, lenticular or lenslet displays and all technologies in which the light scattering surface and the image point are physically separate. Here we present a free-space volumetric display based on photophoretic optical trapping2 that produces full-colour graphics in free space with ten-micrometre image points using persistence of vision. This display works by first isolating a cellulose particle in a photophoretic trap created by spherical and astigmatic aberrations. The trap and particle are then scanned through a display volume while being illuminated with red, green and blue light. The result is a three-dimensional image in free space with a large colour gamut, fine detail and low apparent speckle. This platform, named the Optical Trap Display, is capable of producing image geometries that are currently unobtainable with holographic and light-field technologies, such as long-throw projections, tall sandtables and ‘wrap-around’ displays.
DOI
Combining Holographic Optical Tweezers with Upconversion Luminescence Encoding: Imaging-Based Stable Suspension Array for Sensitive Responding of Dual Cancer Biomarkers
Cheng-Yu Li, Di Cao, Chu-Bo Qi, Ya-Feng Kang, Chong-Yang Song, Dang-Dang Xu, Bei Zheng, Dai-Wen Pang, and Hong-Wu Tang
Establishment of a stable analytical methodology with high-quality results is an urgent need for screening cancer biomarkers in early diagnosis of cancer. In this study, we incorporate holographic optical tweezers with upconversion luminescence encoding to design an imageable suspension array and apply it to conduct the detection of two liver cancer related biomarkers, carcinoembryonic antigen and alpha fetal protein. This bead-based assay is actualized by forming a bead array with holographic optical tweezers and synchronously exciting the upconversion luminescence of corresponding trapped complex beads fabricated with a simple one-step sandwich immunological recognition. Owing to the fact that these flowing beads are stably trapped in the focal plane of the objective lens which tightly converges the array of the laser beams by splitting a 980 nm beam using a diffraction optical element, a fairly stable excitation condition is achieved to provide reliable assay results. By further taking advantage of the eminent encoding capability of upconversion nanoparticles and the extremely low background signals of anti-Stokes luminescence, the two targets are well-identified and simultaneously detected with quite sound sensitivity and specificity. Moreover, the potential on-demand clinical application is presented by employing this approach to respond the targets toward complex matrices such as serum and tissue samples, offering a new alternative for cancer diagnosis technology.
Establishment of a stable analytical methodology with high-quality results is an urgent need for screening cancer biomarkers in early diagnosis of cancer. In this study, we incorporate holographic optical tweezers with upconversion luminescence encoding to design an imageable suspension array and apply it to conduct the detection of two liver cancer related biomarkers, carcinoembryonic antigen and alpha fetal protein. This bead-based assay is actualized by forming a bead array with holographic optical tweezers and synchronously exciting the upconversion luminescence of corresponding trapped complex beads fabricated with a simple one-step sandwich immunological recognition. Owing to the fact that these flowing beads are stably trapped in the focal plane of the objective lens which tightly converges the array of the laser beams by splitting a 980 nm beam using a diffraction optical element, a fairly stable excitation condition is achieved to provide reliable assay results. By further taking advantage of the eminent encoding capability of upconversion nanoparticles and the extremely low background signals of anti-Stokes luminescence, the two targets are well-identified and simultaneously detected with quite sound sensitivity and specificity. Moreover, the potential on-demand clinical application is presented by employing this approach to respond the targets toward complex matrices such as serum and tissue samples, offering a new alternative for cancer diagnosis technology.
Surface Imaging Technique by an Optically Trapped Microsphere in Air Condition
Masaki Michihata, Jonggang Kim, Satoru Takahashi, Kiyoshi Takamasu, Yasuhiro Mizutanim, Yasuhiro Takaya
Recent advancement of fabrication technologies enables nanoscale surface patterning on microstructures, which requires multi-scale measurements for the determination of their geometric dimensions and surface texture in the same coordinate system. Previously, we have developed a micro-coordinate measuring machine with a laser-trapped microprobe that uses an optically trapped microsphere as a surface detecting sensor. In this paper, we propose a surface imaging technique using the optically trapped microsphere as a micro-lens, i.e., the surface imaging system is integrated into the coordinate measuring system. The optically trapped microsphere with a diameter of 8 μm was brought close to a measured surface to image the surface underneath it. Because there is an unknown gap between the microsphere and surface, the focal plane of the imaging system had to be adjusted to the measured surface by the developed imaging lens system. With the microsphere-based imaging system, an optical diffractive grating of sub-micrometer periodic structure with 417 nm pitch and 50 nm depth was successfully imaged and the defect on the grating was detected. Thus, we verified that development of an imaging system based on the optically trapped microsphere for multi-scale evaluation systems can be accomplished.
DOI
Recent advancement of fabrication technologies enables nanoscale surface patterning on microstructures, which requires multi-scale measurements for the determination of their geometric dimensions and surface texture in the same coordinate system. Previously, we have developed a micro-coordinate measuring machine with a laser-trapped microprobe that uses an optically trapped microsphere as a surface detecting sensor. In this paper, we propose a surface imaging technique using the optically trapped microsphere as a micro-lens, i.e., the surface imaging system is integrated into the coordinate measuring system. The optically trapped microsphere with a diameter of 8 μm was brought close to a measured surface to image the surface underneath it. Because there is an unknown gap between the microsphere and surface, the focal plane of the imaging system had to be adjusted to the measured surface by the developed imaging lens system. With the microsphere-based imaging system, an optical diffractive grating of sub-micrometer periodic structure with 417 nm pitch and 50 nm depth was successfully imaged and the defect on the grating was detected. Thus, we verified that development of an imaging system based on the optically trapped microsphere for multi-scale evaluation systems can be accomplished.
DOI
Sliding of a single lac repressor protein along DNA is tuned by DNA sequence and molecular switching
Alessia Tempestini, Carina Monico, Lucia Gardini, Francesco Vanzi, Francesco S Pavone, Marco Capitanio
In any living cell, genome maintenance is carried out by DNA-binding proteins that recognize specific sequences among a vast amount of DNA. This includes fundamental processes such as DNA replication, DNA repair, and gene expression and regulation. Here, we study the mechanism of DNA target search by a single lac repressor protein (LacI) with ultrafast force-clamp spectroscopy, a sub-millisecond and few base-pair resolution technique based on laser tweezers. We measure 1D-diffusion of proteins on DNA at physiological salt concentrations with 20 bp resolution and find that sliding of LacI along DNA is sequence dependent. We show that only allosterically activated LacI slides along non-specific DNA sequences during target search, whereas the inhibited conformation does not support sliding and weakly interacts with DNA. Moreover, we find that LacI undergoes a load-dependent conformational change when it switches between sliding and strong binding to the target sequence. Our data reveal how DNA sequence and molecular switching regulate LacI target search process and provide a comprehensive model of facilitated diffusion for LacI.
DOI
In any living cell, genome maintenance is carried out by DNA-binding proteins that recognize specific sequences among a vast amount of DNA. This includes fundamental processes such as DNA replication, DNA repair, and gene expression and regulation. Here, we study the mechanism of DNA target search by a single lac repressor protein (LacI) with ultrafast force-clamp spectroscopy, a sub-millisecond and few base-pair resolution technique based on laser tweezers. We measure 1D-diffusion of proteins on DNA at physiological salt concentrations with 20 bp resolution and find that sliding of LacI along DNA is sequence dependent. We show that only allosterically activated LacI slides along non-specific DNA sequences during target search, whereas the inhibited conformation does not support sliding and weakly interacts with DNA. Moreover, we find that LacI undergoes a load-dependent conformational change when it switches between sliding and strong binding to the target sequence. Our data reveal how DNA sequence and molecular switching regulate LacI target search process and provide a comprehensive model of facilitated diffusion for LacI.
DOI
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