Reducing photodamage in optical trapping of individual cells in living zebrafish

Panpan Yu, Yifan Liu, Qian Zhao, Ziqiang Wang, Yinmei Li and Lei Gong

We propose to reduce the photodamage in optical trapping of living cells by using azimuthally polarized beams (APBs). APBs achieve higher axial trapping efficiency and lower photodamage than linearly polarized Gaussian beam when used for optical trapping of individual red blood cells (RBCs). In particular, we further achieve reducing photodamage in optical trapping of individual RBCs in living Zebrafishes. Our work is expected to benefit in-vivo optical trapping and the study of living cells.

DOI